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![[Figure 3]](rr5256fig3mag.jpg)
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Figure 3
2DTM is sensitive to pixel-size errors of ∼0.2%. (a, b) Projection images from the same orientation of the LSU template with different pixel sizes: 0.95 Å (a) or 0.93 Å (b). An ∼2% difference causes significant changes in the size of template projections. (c) Intensity plot showing the difference in the intensity between (a) and (b), with each projection normalized to a mean of zero and a standard deviation of 1, showing a large change in intensity from 0.95 to 0.93 Å. (d) Histogram of the mean optimal pixel size in each micrograph with more than 50 LSU detections above a z-score of 8. The box plot shows the interquartile range and the whiskers represent the 5th and 95th percentiles. The mean and 95% confidence intervals of these pixel sizes are also plotted. 90% of the data were <0.2% from the mean. (e) A scatter plot showing the average reduction in z-score relative to the maximum for incorrect pixel sizes. The error bars are 95% confidence intervals. A sum of two Gaussian distributions is fitted to the data. The difference in pixel size shown in (a) and (b) causes a ∼20% decrease in 2DTM z-scores. |
![[Figure 3]](rr5256fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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