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![[Figure 5]](rr5256fig5mag.jpg)
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Figure 5
Constrained searches allow the identification of a continuous distribution of conformational states. (a) A histogram showing the angular distribution of SSU rotations relative to the LSU, as determined by constrained 2DTM. (b) A histogram showing the angular distribution of SSU at a secondary axis (subunit roll) relative to the LSU, as determined by constrained 2DTM. (c) A 2D heatmap combining the results shown in (a) and (b), where the intensity corresponds to the occupancy of SSUs. (d) Reconstructions of LSU particles classified by the output of 2DTM as having a rotated (≥4) or nonrotated (<4) SSU. (e) Reconstructions as in (d) where features within 5 Å of tRNAs and eEF1A (shown as ribbon diagrams) are highlighted as opaque by docking PDB entry 8z71 (Cheng et al., 2025  ; blue) into the `nonrotated' density (cyan) or PDB entry 8xu8 (Cheng et al., 2025; pink) into the `rotated' density (magenta). |
![[Figure 5]](rr5256fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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