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![[Figure 3]](ag5062fig3mag.jpg)
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Figure 3
Activity of SmCD1. (a) SmCD1 activity measured over time under different pH conditions, recording the fluorescence of the fluorogenic substrate 7-methoxycoumarin-4-acetyl-GKPILFFRLK(DNP)-D-R-amide. (b) SmCD1 activity at pH 3.5, incubated with 200 nM Nb10C9 or pepstatin. Data were background-subtracted and are displayed as the mean of n = 3 repeats. (c) Estimation plot of unpaired Student's t-test for activity data of 50 nM SmCD1 or 50 nM SmCD1 + 200 nM Nb10C9 at the point of enzyme saturation, between 3000 and 4000 s. (d) Titration ELISA of Nb10C9 bound to 50 nM SmCD1 between pH 3.0 and pH 7.5. Data were background-subtracted and a line of best fit is displayed. |
![[Figure 3]](ag5062fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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