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Figure 3
Cryo-EM data-processing workflow for separating GroEL contaminants from ATAD2B particles. (a) Initial 2D class averages from blob picking show predominantly heptameric GroEL particles (top). Further inspection reveals hexameric ATAD2B classes (marked with green dots, middle). A representative micrograph indicates the presence of both GroEL (blue circles) and ATAD2B particles (cyan circles). (b) Topaz was trained to identify GroEL particles in raw micrographs. (c) Topaz extraction recovered more ATAD2B particles than expected. 2D class averages display GroEL (top row) and ATAD2B particles (middle and bottom rows) within the same data set. Heterogenous refinement was used to separate the ATAD2B and GroEL particles, yielding a final ATAD2B map at 5.1 Å resolution from 45 889 particles.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 3| March 2026| Pages 253-273
https://doi.org/10.1107/S205979832600080X
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