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![[Figure 5]](ai5014fig5mag.jpg)
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Figure 5
Single-particle cryo-EM data-processing scheme for the GroEL–γATP complex. A total of 317 080 GroEL particles were selected following 2D classification and subjected to ab initio reconstruction into six classes. Three of these ab initio classes were retained for further processing and used to generate a homogeneous 3D map. Subsequent 3D classification yielded four classes, with one class identified as junk and excluded from further analysis. The remaining three classes were combined, resulting in a final reconstruction comprising 261 106 particles with a CryoSPARC gold-standard FSC resolution of 2.95 Å; however, we report the no-mask resolution of 3.3 Å for the final map. Local resolution estimation was performed in CryoSPARC and the resulting map was visualized using UCSF ChimeraX. |
![[Figure 5]](ai5014fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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