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![[Figure 6]](ai5014fig6mag.jpg)
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Figure 6
Single-particle cryo-EM data-processing scheme for the apo GroEL complex. A total of 148 187 GroEL particles were selected after 2D classification and subjected to ab initio reconstruction. Homogeneous refinement was performed on the resulting single class. Subsequent 3D classification yielded five classes; adopting a conservative approach, only the highest quality class was selected for final map generation. The final reconstruction comprised 42 776 particles and had a final resolution of 3.32 Å according to the CryoSPARC gold-standard FSC. However, we report our final map as 3.7 Å resolution with no mask applied. Local resolution estimation was performed in CryoSPARC and the resulting map was visualized using UCSF ChimeraX. |
![[Figure 6]](ai5014fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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