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![[Figure 7]](ai5014fig7mag.jpg)
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Figure 7
Single-particle cryo-EM data-processing scheme for the GroEL–ADP complex. The particle stack, comprising 125 716 GroEL particles selected from a 2D classification in cisTEM, was exported for further processing in FREALIGN. An initial alignment was performed using a low-pass filtered GroEL template. Subsequently, the aligned particles underwent 3D classification over 50 rounds to sort them into distinct conformational states. However, no major confirmational changes were observed in the three best classes. These classes were combined to generate a final map at 4.2 Å resolution. The two half-maps generated from the final 3D reconstruction were used to compute a Fourier shell correlation (FSC) curve by using the EMDB validation tool (https://www.ebi.ac.uk/emdb/validation/fsc/). |
![[Figure 7]](ai5014fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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