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![[Figure 4]](gm5116fig4mag.jpg)
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Figure 4
Apoferritin single-particle data analysis and final resolution estimation. (a) Complete electron-density map of apoferritin from 6488 particles without X-ray exposure. (b) The Fourier shell correlation analysis of the three maps yielded resolutions of 3.17 Å for the dataset without X-ray exposure and 3.56 and 3.88 Å for the apoferritin samples which had absorbed 1 and 100 MGy X-ray doses, respectively. All three reconstructions were based on the same number of particles. These results indicate that higher X-ray exposure doses caused progressive protein damage, leading to a loss of resolution. (c) The apoferritin model (PDB entry 6rjh; Naydenova et al., 2019  ) was fitted into all three maps to illustrate that each electron-density map distinctly reveals the backbone and side-chain densities, even though the side-chain densities in the 100 MGy map appear noticeably weaker. |
![[Figure 4]](gm5116fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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