An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM

Marchán Torres, D.; Conesa, P.; Garcia, A.; Iceta, M.; Broche, L.; Gragera, M.; Linares, R.; Kwong, H.S.; Chichón, F.J.; Svensson, O.; Arranz, R.; Effantin, G.; Kandiah, E.; Carazo, J.M.; Sorzano, C.O.S.

doi:10.1107/S205979832600656X

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 5
Detailed Scipion workflow diagram for the refinement and parallel validation stage. After pruning particles during early 3D classification, particles from the most populated class are analyzed in two concurrent processing branches: the 2D branch and the 3D branch. Results from both branches are cross-validated (consistency check) to select the optimal particle set for final refinement. Sky-blue labels correspond to image-processing steps for the 2D branch, while purple labels denote those for the 3D branch. Automated decisions are indicated with green labels, highlighting critical steps for this stage.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 82| Part 8| August 2026| Pages 915-939

https://doi.org/10.1107/S205979832600656X

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