An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM

Marchán Torres, D.; Conesa, P.; Garcia, A.; Iceta, M.; Broche, L.; Gragera, M.; Linares, R.; Kwong, H.S.; Chichón, F.J.; Svensson, O.; Arranz, R.; Effantin, G.; Kandiah, E.; Carazo, J.M.; Sorzano, C.O.S.

doi:10.1107/S205979832600656X

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 7
Suboptimal cases: each entry corresponds to one of five datasets in which the final 3D reconstruction was classified as suboptimal. For each case, the deposited EMDB volume, the reconstructed 3D map (raw, unsharpened reconstruction) and the specific issues identified by the pipeline are shown, highlighting the reasons for suboptimal reconstruction quality.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 82| Part 8| August 2026| Pages 915-939

https://doi.org/10.1107/S205979832600656X

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