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Figure 6
Segmentation and subtraction of diverse membrane mimics and curved membranes. (a) LocScale-FEM maps of MsbA solved in 12 different membrane mimics (EMDB entries EMD-50774 to EMD-50794) before (blue) and after (grey) segmentation and micelle subtraction with SURFER. Arrows indicate retained density of membrane-scaffold proteins, peptidiscs and amphipols. (b) Segmentation of curved membrane tubes for EMDB entry EMD-20588. SURFER segmentations are shown for default settings and after optimization; the subtracted map corresponds to the optimized segmentation

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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