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![[Figure 3]](sor5006fig3mag.jpg)
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Figure 3
(a) Runtime of LowDoseWizard. Dashed horizontal lines represent the mean runtime of each routine. Horizontal transparent bars represent the respective standard deviation and violin plots represent the raw data (optics, n = 67; camera, n = 65; image shift, n = 67; tuning, n = 45). (b) Gold-standard FSC plot of half-maps from the Krios (cyan) and Glacios (orange) data sets. The grey vertical line indicates the Nyquist frequency of the Glacios data set. The dashed vertical line indicates the physical Nyquist frequency of the Krios data set. FSCs were calculated with relion_postprocess. (c) Densities from the Glacios data (level 0.2). (d) Densities from the Krios data of same residues as in (c) shown at low (grey surface, level 0.2) and high (magenta mesh, level 0.52) thresholds. PDB entry 7a4m was fitted into all densities. All densities were generated with relion_postprocess. (e) Rosenthal–Henderson B-factor plot (Rosenthal & Henderson, 2003  ) of the Krios (cyan) and Glacios (orange) data sets. Error bars represent the standard deviation of the resolution estimation from the random resampling and the number in parentheses represents the standard deviation of the fitted B factor. The respective data point of the full particle stack (not included in the straight-line fit) is highlighted by dashed lines. |
![[Figure 3]](sor5006fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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