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Figure 1
(a) Microphotograph of NvFdhAB microcrystals, approximately 30 × 20 × 5 µm in size, in suspension in mother liquor. (b) Superposition of the RT-SSX and cryo NvFdhAB (PDB entry 6sdr) structures in violet and black, respectively. The W site with the two MGD cofactors and the four [4Fe–4S] clusters shown in ball-and-stick representation. (c, d, e) Examples of the differences in side-chain positioning and hydration water location between the superimposed SSX and cryo NvFdhAB (PDB entry 6sdr) structures in violet and black, respectively, around residues Tyr185 and Arg263, Phe160 and Trp533 and, lastly, Met209. In (c) the absent water molecule is shown as a burgundy sphere. Hydrogen bonds are shown as teal dashes and distances are in Å. RT-SSX 2mFoDFc electron-density maps are shown as a blue mesh at 1.0 r.m.s.d.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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