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![[Figure 1]](eh5026fig1mag.jpg)
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Figure 1
Experimental setup for plunge-free cryoEM grid preparation. Components are numbered as follows: 1, swinging arm; 2, grid holder; 3, nitrogen/ethane cup; 4, stage; 5, stepper motor; 6, arm limit switches; 7, syringe pump; 8, camera; 9, LED light source; 10, tweezers; 11, grid; 12, spray nozzle. (a) Overview of the device. The red dashed box indicates the region shown in (b). (b) Magnified front view showing the swinging arm, ethane cup and grid before and after sample application (left and right, respectively). The red dashed arrow (right) indicates the direction of movement of the arm and the red asterisk highlights the disturbance of the liquid ethane surface. (c) Magnified side view of the swinging arm before and after sample application (top and bottom, respectively). The red asterisk (bottom) highlights the disturbance of the liquid ethane surface. |
![[Figure 1]](eh5026fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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