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Figure 2
Low-magnification electron micrographs and tomographic reconstructions of the deposited thin sheets of vitreous ice. (a) Grid overview indicating the approximate direction of the spray nozzle with a dashed arrow. The scale bar corresponds to 100 µm. (b) Higher magnification images of the areas of ice suitable for imaging, highlighted with red asterisks. (c) Tomographic reconstructions of the areas shown in (b) with the grid surface as a grey slice and the supporting ice structure in dark blue, and indicating the position of the thin ice sheets in transparent blue (marked with red asterisks). Note that due to the large contour difference between the thick and thin areas the areas of thin ice are not visible at the contour level and are therefore represented by the area in transparent blue.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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