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![[Figure 1]](nw5141fig1mag.jpg)
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Figure 1
Graphical representation of the Global Phasing Ltd (GPhL) workflow (Fogh et al., 2026  ). The GPhL data-collection workflow starts with crystal characterization (step 1). For this, 1.2° thin wedges are collected at 0°, 45°, 90° and 180° with ω-slicing of 0.1° at the PETRA III P14 beamline. These images are indexed using XDS, the apparent space group determined and a strategy is calculated to collect data covering the Bragg sphere in reciprocal space in an optimal manner, while avoiding collisions and goniostat shadows. These require the user to specify detector distance, a dose budget and a radiation-sensitivity factor. The data are collected according to a multi-orientation strategy computed by the workflow software, making use of the capabilities of the multi-axis goniostat. Each time the crystal is reoriented by the κ and φ axes (step 2), the user is required to recentre the crystal. The data are then processed in an automated manner using the aP_wf_process interface to autoPROC (step 3). |
![[Figure 1]](nw5141fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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