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Figure 2
Changes in the unit-cell volume of ferritin crystals with temperature and absorbed X-ray dose. (a) Holoferritin constant series 1 (HCS_1) and holoferritin temperature series 1 (HTS_1) recorded on ID23-1 at ESRF. (b) Apoferritin constant series 1 (ACS_1) recorded on ID14-4 at ESRF. (DS-7 is missing because the images were not recorded.) The individual datasets were collected over 80 s at 20% transmission and the burns with unattenuated beam between each dataset for varying times: 4 s (burns after DS1 and DS2), 5 s (DS3 and DS4), 10 s (DS5, DS6 and DS7), 20 s (DS8 and DS9), 25 s (DS10 and DS11), 25 s (DS12), 35 s (DS13), 45 s (DS14) and 35 s (DS15). These increasing burn times account for the non-even spacing in dose of the dataset points above. (c) Apoferritin temperature series 1 (ATS_1) recorded on ID14-4 at ESRF. The purpose of the solid lines is to guide the eye; they are not fits. Linear extrapolation of the first four 100 K unit-cell volumes to 2.0 MGy falls below the later 100 K points, whereas if this extrapolation is restricted to the third and fourth 100 K values, it appears to approach the later values much more closely. The purpose of the solid lines is to guide the eye; they are not fits.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 10| October 2026|
https://doi.org/10.1107/S2059798326008612