Radiation damage in serial femtosecond crystallography studied in hemoglobin

Galchenkova, M.; Dawod, I.; Cardoch, S.; Sprenger, J.; Oberthür, D.; Awel, S.; De Santis, E.; Grånäs, O.; Knoska, J.; Metz, M.; Passmore, S.; Pena, G.; Sarrou, I.; Wiedorn, M.; Timneanu, N.; Chapman, H.; Yefanov, O.; Caleman, C.

doi:10.1107/S2059798326008569

Figure 1
(a) Schematic structure of tetrameric (α₂β₂) hemoglobin highlighting the positions of the heme groups. (b) Overlay of structures of horse hemoglobin refined against data collected with 3 fs (cyan cartoon) and 10 fs (brown cartoon) XFEL pulse durations. The β-subunits are in darker tones compared with the corresponding α-subunits. The heme groups are represented as sticks with carbon in the respective color of the backbone (brown or cyan), oxygens in red, nitrogens in blue and the central iron as an orange sphere.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 82| Part 10| October 2026| Pages 1169-1180

https://doi.org/10.1107/S2059798326008569

Open

access