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Figure 5
Electron tomography of mitochondria in a U2OS cell sample using the updated SavvyScan and post-processing by the shadow montage technique. (a) Overview of the grid generated by SerialEM from a montage of low-magnification HAADF scans. (b) One grid-square scan controlled by SerialEM. (c) Direct HAADF image of one projection. (d) Post-processing a shadow montage based on the 4D-STEM dataset of one tilt view. (e) A tomogram built from 37 tilt views in shadow montage (labels: DM, double membrane; G, calcium phosphate granule; C, cristae).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 10| October 2026| Pages 1203-1211
https://doi.org/10.1107/S2059798326009630