CsMT: a robust and streamlined CryoSPARC workflow for cryo-EM reconstruction of microtubules

Alagha, T.; Arin, A.; Vangos, N.; Goodey-Parfitt, H.; Ngo, H.N.; Dau, N.N.; Nguyen, M.H.; Legal, T.; Cianfrocco, M.A.; Bui, K.H.

doi:10.1107/S2059798326010144

Figure 3
Principle of classification and grouping for 1-PF and 2-PF pair reconstruction. For clarity, a 2 × 2 tubulin dimer subtraction mask is shown here, but a 3 × 2 mask is preferred as it provides more signal for classification.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 82| Part 11| November 2026| Pages 1212-1223

https://doi.org/10.1107/S2059798326010144

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