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Figure 5
The CsMT workflow for decorated MT. (a) If the MT is fully decorated, the tubulin classification step in CsMT from the α/β-averaged map can also include the decorated MAP by making the classification mask also contain the 4 nm averaged MAP density. (b) If the MT is partially decorated, the tubulin classification step in CsMT is performed strictly based on the tubulin mask and the synthetic references of α-first and β-first PF. After that, the correctly 8 nm registered PF map is subjected to another classification to separate the decorated PF particles from the undecorated PF particles. (c) PF map of tubulin decorated with KIF5A, EMDB entry EMD-78245, reconstructed from EMPIAR-13372 (Zhang et al., 2026View full citation) at 2.6 Å resolution.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 82| Part 11| November 2026| Pages 1212-1223
https://doi.org/10.1107/S2059798326010144