most read articles
These are the most read articles in the last six months.
Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules. (2022). Acta Cryst. D78, 1337-1346.
Protein structure prediction by AlphaFold2: are attention and symmetries all you need?. (2021). Acta Cryst. D77, 982-991.
Time-resolved serial femtosecond crystallography on fatty-acid photodecarboxylase: lessons learned. (2022). Acta Cryst. D78, 1131-1142.
CCP4 Cloud for structure determination and project management in macromolecular crystallography. (2022). Acta Cryst. D78, 1079-1089.
Putting AlphaFold models to work with phenix.process_predicted_model and ISOLDE. (2022). Acta Cryst. D78, 1303-1314.
Probing ligand binding of endothiapepsin by `temperature-resolved' macromolecular crystallography. (2022). Acta Cryst. D78, 964-974.
Determining the oxidation state of elements by X-ray crystallography. (2022). Acta Cryst. D78, 238-247.
The crystal structure of CbpD clarifies substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases. (2022). Acta Cryst. D78, 1064-1078.
Theoretical 3D electron diffraction electrostatic potential maps of proteins modeled with a multipolar pseudoatom data bank. (2022). Acta Cryst. D78, 1010-1020.
Dependence of crystallographic atomic displacement parameters on temperature (25–150 K) for complexes of horse liver alcohol dehydrogenase. (2022). Acta Cryst. D78, 1221-1234.
Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion. (2022). Acta Cryst. D78, 883-889.
Native glycosylation and binding of the antidepressant paroxetine in a low-resolution crystal structure of human myeloperoxidase. (2022). Acta Cryst. D78, 1099-1109.
Training undergraduate research assistants with an outcome-oriented and skill-based mentoring strategy. (2022). Acta Cryst. D78, 936-944.
Cryo-EM single-particle structure refinement and map calculation using Servalcat. (2021). Acta Cryst. D77, 1282-1291.
High-resolution structure determination using high-throughput electron cryo-tomography. (2022). Acta Cryst. D78, 817-824.
Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre. (2022). Acta Cryst. D78, 698-708.
Fast fragment- and compound-screening pipeline at the Swiss Light Source. (2022). Acta Cryst. D78, 328-336.
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. (2022). Acta Cryst. D78, 1120-1130.
Over the rainbow: structural characterization of the chromoproteins gfasPurple, amilCP, spisPink and eforRed. (2022). Acta Cryst. D78, 599-612.
A GH115 α-glucuronidase structure reveals dimerization-mediated substrate binding and a proton wire potentially important for catalysis. (2022). Acta Cryst. D78, 658-668.
Exiting the tunnel of uncertainty: crystal soak to validated hit. (2022). Acta Cryst. D78, 1294-1302.
On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy. (2022). Acta Cryst. D78, 410-423.
Elucidation of protein function using computational docking and hotspot analysis by ClusPro and FTMap. (2022). Acta Cryst. D78, 690-697.
Application of sulfur SAD to small crystals with a large asymmetric unit and anomalous substructure. (2022). Acta Cryst. D78, 1021-1031.
MrParse: finding homologues in the PDB and the EBI AlphaFold database for molecular replacement and more. (2022). Acta Cryst. D78, 553-559.
Protein dynamics developments for the large scale and cryoEM: case study of ProDy 2.0. (2022). Acta Cryst. D78, 399-409.
Structural basis of human LRG1 recognition by Magacizumab, a humanized monoclonal antibody with therapeutic potential. (2022). Acta Cryst. D78, 725-734.
ModelCraft: an advanced automated model-building pipeline using Buccaneer. (2022). Acta Cryst. D78, 1090-1098.
Implications of AlphaFold2 for crystallographic phasing by molecular replacement. (2022). Acta Cryst. D78, 1-13.
Development of CryoVR, a virtual reality training system for hands-on cryoEM operations. (2022). Acta Cryst. D78, 903-910.
Knowledge-based prediction of DNA hydration using hydrated dinucleotides as building blocks. (2022). Acta Cryst. D78, 1032-1045.
How do I get the most out of my protein sequence using bioinformatics tools?. (2021). Acta Cryst. D77, 1116-1126.
Sequence-assignment validation in cryo-EM models with checkMySequence. (2022). Acta Cryst. D78, 806-816.
Pivotal role of a conserved histidine in Escherichia coli ribonuclease HI as proposed by X-ray crystallography. (2022). Acta Cryst. D78, 390-398.
Detecting ice artefacts in processed macromolecular diffraction data with machine learning. (2022). Acta Cryst. D78, 187-195.
Best practices for time-resolved serial synchrotron crystallography. (2022). Acta Cryst. D78, 14-29.
Crystal structure of the putative cell-wall lipoglycan biosynthesis protein LmcA from Mycobacterium smegmatis. (2022). Acta Cryst. D78, 494-508.
Structural basis for substrate recognition in the Phytolacca americana glycosyltransferase PaGT3. (2022). Acta Cryst. D78, 379-389.
Highlighting the factors governing transglycosylation in the GH5_5 endo-1,4-β-glucanase RBcel1. (2022). Acta Cryst. D78, 278-289.
Global fitting of multiple data frames from SEC–SAXS to investigate the structure of next-generation nanodiscs. (2022). Acta Cryst. D78, 483-493.
Molecular-dynamics simulation methods for macromolecular crystallography. (2023). Acta Cryst. D79, 50-65.
Updated restraint dictionaries for carbohydrates in the pyranose form. (2022). Acta Cryst. D78, 455-465.
Structural snapshot of a glycoside hydrolase family 8 endo-β-1,4-glucanase capturing the state after cleavage of the scissile bond. (2022). Acta Cryst. D78, 228-237.
A recurring packing contact in crystals of InlB pinpoints functional binding sites in the internalin domain and the B repeat. (2022). Acta Cryst. D78, 310-320.
Raw diffraction data are our ground truth from which all subsequent workflows develop. (2022). Acta Cryst. D78, 683-689.
Structural and functional analysis of the D-alanyl carrier protein ligase DltA from Staphylococcus aureus Mu50. (2022). Acta Cryst. D78, 424-434.
Structural bases for the higher adherence to ACE2 conferred by the SARS-CoV-2 spike Q498Y substitution. (2022). Acta Cryst. D78, 1156-1170.
Inhibition of Schistosoma mansoni carbonic anhydrase by the antiparasitic drug clorsulon: X-ray crystallographic and in vitro studies. (2022). Acta Cryst. D78, 321-327.
Synergic action of an inserted carbohydrate-binding module in a glycoside hydrolase family 5 endoglucanase. (2022). Acta Cryst. D78, 633-646.
Structural insights into choline-O-sulfatase reveal the molecular determinants for ligand binding. (2022). Acta Cryst. D78, 669-682.
CSSR: assignment of secondary structure to coarse-grained RNA tertiary structures. (2022). Acta Cryst. D78, 466-471.
A simple technique to classify diffraction data from dynamic proteins according to individual polymorphs. (2022). Acta Cryst. D78, 268-277.
Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography. (2021). Acta Cryst. D77, 1251-1269.
Molecular insight into 2-phosphoglycolate activation of the phosphatase activity of bisphosphoglycerate mutase. (2022). Acta Cryst. D78, 472-482.
Using deep-learning predictions of inter-residue distances for model validation. (2022). Acta Cryst. D78, 1412-1427.
Protein model refinement for cryo-EM maps using AlphaFold2 and the DAQ score. (2023). Acta Cryst. D79, 10-21.
A case for glycerol as an acceptable additive for single-particle cryoEM samples. (2022). Acta Cryst. D78, 124-135.
Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography. (2021). Acta Cryst. D77, 628-644.
Using photocaging for fast time-resolved structural biology studies. (2021). Acta Cryst. D77, 1218-1232.
Verification: model-free phasing with enhanced predicted models in ARCIMBOLDO_SHREDDER. (2022). Acta Cryst. D78, 1283-1293.
Crystallographic and X-ray scattering study of RdfS, a recombination directionality factor from an integrative and conjugative element. (2022). Acta Cryst. D78, 1210-1220.
Zymogenic latency in an ∼250-million-year-old astacin metallopeptidase. (2022). Acta Cryst. D78, 1347-1357.
Optimized cryo-EM data-acquisition workflow by sample-thickness determination. (2021). Acta Cryst. D77, 565-571.
Structural features of chloroplast trigger factor determined at 2.6 Å resolution. (2022). Acta Cryst. D78, 1259-1272.
Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion. (2021). Acta Cryst. D77, 645-662.
Frag4Lead: growing crystallographic fragment hits by catalog using fragment-guided template docking. (2021). Acta Cryst. D77, 1168-1182.
Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8. (2022). Acta Cryst. D78, 1373-1383.