Big changes are ahead – a new format for crystallization communications in Acta Cryst. F

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Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana

The X-ray structure of the light, oxygen or voltage domain 2 of phototropin 1 from A. thaliana (AtLOV2) in its dark-adapted state has been determined. The N-terminal flanking A′α helix of AtLOV2 plays a role in its dimerization.

Structure of the secretion domain of HxuA from Haemophilus influenzae

The structure of the secretion domain of HxuA from H. influenzae has been determined to 1.5 Å resolution.

Structure of the uracil complex of Vaccinia virus uracil DNA glycosylase

The crystal structure of the uracil complex of Vaccinia virus uracil DNA glycosylase (D4) has been determined at 2.03 Å resolution.

Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution

The 1.82 Å resolution X-ray structure of a thermally tolerant glycoside hydrolase family 74 enzyme from A. cellulolyticus is reported.

Two high-resolution structures of the human E3 ubiquitin ligase Siah1

Two structures of human Siah1 at 1.95 and 1.58 Å resolution provide more complete models for this protein and identify conformational variability in the subdomain organization.

Structure of the β-form of human MK2 in complex with the non-selective kinase inhibitor TEI-L03090

The glycine-rich loop of MK2 (MAPKAP-K2) bound to the non-selective inhibitor TEI-L03090 adopts the β-form, which is commonly found in other kinase complexes; however, it differs from the α-form which is adopted when MK2 is bound to the MK2-selective inhibitor TEI-I01800. These results show how conformational change of MK2 influences its selectivity.

Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG

Structural insights into the type-VIII protein secretion system are described.

Purification, crystallization and preliminary X-ray studies of MbtN (Rv1346) from Mycobacterium tuberculosis

MbtN (Rv1346) from M. tuberculosis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 2.3 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase type I

Human peptidylarginine deiminase type I was crystallized using polyethylene glycol as a precipitant. The crystals belonged to space group P6₁, with unit-cell parameters a = b = 90.3, c = 372.3 Å, and contained two molecules in the asymmetric unit.

Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chito­tetraose

A selenomethionine-labelled active-site mutant of `loopless' family GH19 chitinase from B. coronatum has been co-crystallized with chitotetraose. X-ray diffraction data were collected to 1.58 Å resolution using a synchrotron-radiation source.

Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre

The trimeric L-shaped fibre protein from bacteriophage T5 has been analysed by electron microscopy and a C-terminal domain has been crystallized. Diffraction data have been obtained for two native crystal forms and one selenomethionine derivative.

Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria

Two-dimensional crystallization conditions for intact bovine F-ATP synthase have been established.

Crystallization and preliminary crystallographic analysis of the chimeric protein LKB1–14-3-3ζ

The chimeric protein LKB1–14-3-3ζ was purified and crystallized. The X-ray diffraction were collected to a resolution of 2.9–Å.

Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus

A C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus, has been crystallized. Diffraction data have been obtained to high resolution for several native crystal forms and a selenomethionine derivative.

Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella

Recombinant SAG19 from E. tenella has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.

Crystallization and preliminary X-ray analysis of the ATPase domain of the σ⁵⁴-dependent transcription activator NtrC1 from Aquifex aeolicus bound to the ATP analog ADP–BeF_x

This study reports the crystallization of a new nucleotide state of the ATPase domain of a bacterial transcription activator NtrC1 from the hyperthermophilic bacterium Aquifex aeolicus. Wild-type NtrC1 ATPase domain was crystallized in the presence of the ATP analog ADP–BeF_x–Mg and the crystals diffracted anisotropically to at best 3.2, 5.2 and 3.2 Å resolution in the a*, b* and c* directions, respectively.

Crystallization and sulfur SAD phasing of AggA, the major subunit of aggregative adherence fimbriae type I from the Escherichia coli strain that caused an outbreak of haemolytic-uraemic syndrome in Germany

Donor-strand complemented AggA subunit of aggregative adherence fimbriae type I from Shiga toxin-producing E. coli O104:H4 was crystallized. Diffraction data were collected to 1.55 Å resolution. Initial phases were derived from a sulfur single anomalous diffraction experiment.

Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K

The N-terminal cysteine, histidine-dependent amidohydrolase (peptidase) domain of the S. aureus bacteriophage K endolysin LysK has been crystallized. Native and derivative data to a resolution of 1.7–1.8 Å have been obtained, which allowed structure solution.

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

The class B carbapenemase IMP-18 from P. aeruginosa was overexpressed in E. coli, purified and crystallized. The crystals belonged to space group P4₁2₁2 and diffracted to 2 Å resolution.

Preliminary crystallographic studies of BRCA1 BRCT–ABRAXAS complex

The crystallization and preliminary X-ray diffraction analysis of BRCA1 BRCT with ABRAXAS phosphopeptide are reported. Binding affinity using ITC has been compared.

Liquid–liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetyl­glucosaminidase from Streptococcus pyogenes with activity on human IgG

A comparative study of vapor diffusion versus liquid–liquid diffusion methods used for the crystallization of EndoS is reported. X-ray diffraction data to 2.6 and 1.9 Å resolution were collected for wild-type endoglycosidase and glycosynthase constructs of EndoS, respectively.

Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1

A crystal of Tm-1, which is an inhibitor protein of Tomato mosaic virus RNA replication, was obtained using the hanging-drop vapour-diffusion method at 293 K and diffracted X-rays to 2.7 Å resolution. It belonged to space group P1, with unit-cell parameters a = 77.97, b = 105.28, c = 110.62 Å, α = 94.6, β = 109.3, γ = 108.0°.

Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus

The (R)-selective amine transaminase from A. fumigatus was purified and crystallized. Diffraction data to 1.84 Å resolution for S-SAD phasing as well as high-resolution data to 1.27 Å were collected from a single crystal belonging to space group C222₁.

Expression, purification, crystallization and preliminary X-ray analysis of the receiver domain of Staphylococcus aureus LytR protein

The expression, purification, crystallization and preliminary X-ray diffraction analysis of the N-terminal receiver domain of LytR, LytR^N, from S. aureus is reported.

Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis

Argininosuccinate lyase from M. tuberculosis has been cloned, purified and crystallized, and preliminary X-ray studies on the crystals have been carried out.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD

The recombinant aspartate transcarbamoylase domain of human CAD was expressed in E. coli, purified and crystallized in the presence and absence of the inhibitor PALA. X-ray diffraction data sets were collected for both crystal forms at 2.1 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids

To determine the molecular basis underlying the ligand specificity of the ligand-binding regions of Pseudomonas chemotactic transducer A and B, complexes with amino acids have been purified and crystals have been produced.

Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein

The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. cAMP-free D53H CRP crystals were obtained and diffracted at a resolution of 2.9 Å. Based on the systematic absences of the crystals, the likely space group is P2₁2₁2₁ with the unit-cell parameters a = 76.66, b = 152.14, and c = 176.11 Å. The asymmetric unit was confirmed to contain four protein dimers with a Matthews coefficient of 2.71 ų Da⁻¹ and a solvent content of 54.68%.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylano­hydrolase (Xyn30A) from Clostridium thermocellum

The glycoside hydrolase family 30 module from the cellulosomal C. thermocellum XynGH30 was purified and crystallized and data were collected to 1.4 Å resolution.