Pteridine glycosyltransferase from Chlorobium tepidum: crystallization and X-ray analysis

The cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of pteridine glycosyltransferase from Chlorobium tepidum are reported.


Introduction
Tetrahydrobiopterin (BH 4 ) is an essential cofactor for enzymes involved in neurotransmitter biosynthesis, hydroxylation of aromatic amino acids (phenylalanine hydroxylase and tryptophan hydroxylase) and nitric oxide (NO) synthesis (Werner et al., 2011) in mammals. It also contributes to the proliferation of hematopoietic cells and mammalian cell lines (Thö ny et al., 2000;Nagatsu & Ichinose, 1999). In addition, BH 4 may have a role in endotheliumdependent vasodilation in atherosclerosis, diabetes mellitus and vascular dysfunctions of chronic smokers (Hashimoto et al., 2004). It has been reported that a glycosylated form of BH 4 is present in some prokaryotes such as Sulfolobus solfataricus and Chlorobium tepidum, and abundantly in cyanobacteria including Synechococcus PCC7942, Nostoc sp. and Synechocystis sp. (Chung et al., 2000). In these bacteria, there are a group of enzymes called pteridine glycosyltransferases (PGTs) which catalyse the transfer of sugar moieties from activated donor molecules such as UDP-glucose, UDP-xylose and UDPgalactose to specific pteridine acceptor molecules including BH 4 , biopterin and neopterin to produce various pteridine glycosides (Wachi et al., 1995).
To understand the structure and mechanism of CtPGT, we isolated the corresponding gene from C. tepidum and cloned it for expression in E. coli. CtPGT was expressed, purified and crystallized for structural studies. A description of the expression, purification, crystallization and X-ray diffraction analysis of CtPGT is given below.

Macromolecule production
The gene for pteridine glycosyltransferase from C. tepidum (CtPGT) was isolated and amplified by polymerase chain reaction using primers with NcoI and KpnI restriction-enzyme sites (Table 1; the sites are underlined in the primers). The double-digested PCR product and the pProEX HTa vector (Life Technologies, Carlsbad, California, USA) were mixed in different ratios for ligation at 289 K overnight. The ligated product was then transformed into XL1-Blue cells. Several colonies were selected, and insertion of the DNA fragment was checked by colony PCR and restriction-enzyme digestion with NcoI and KpnI. The insertion of the CtPGT gene into the expression vector was further confirmed by DNA sequencing.
The cloned expression plasmid carrying the CtPGT gene was transformed into E. coli strain BL21(DE3) cells for protein expression. An initial culture of 100 ml LB broth (10 g bactotryptone, 5 g yeast extract and 10 g NaCl per litre of solution) with 0.1 mg ml À1 ampicillin was seeded with a single colony. This starter culture was incubated at 310 K with vigorous shaking at 180 rev min À1 overnight. From the overnight culture, 20 ml of the starter culture was used to inoculate 1000 ml M9 medium (6 g Na 2 HPO 4 , 3 g KH 2 PO 4 , 1 g NH 4 Cl, 0.5 g NaCl, 2 g glucose, 2 mM MgSO 4 and 0.1 mM CaCl 2 per litre of Milli-Q water) in the presence of ampicillin (0.1 mg ml À1 ). All of the essential amino acids including selenomethionine were supplied externally in M9 medium. The cells were then grown at 310 K with shaking at 180 rev min À1 until the optical density (OD 600 ) reached 0.6, and 0.4 mM isopropyl -d-1-thiogalactopyranoside (IPTG) was subsequently injected to induce protein expression. After adding IPTG, the bacterial culture was shifted to 303 K and grown with shaking at 180 rev min À1 overnight. On the morning of the next day, the cells were harvested by centrifugation at 277 K at 6520g for 10 min. The pellet was resuspended in 80 ml binding/lysis buffer consisting of 50 mM phosphate pH 8.0, 500 mM NaCl, 5 mM -mercaptoethanol and disrupted by sonication for 5 min with 3 s pulse and 30% amplitude. It was then centrifuged at 15 930g and 277 K for 30 min. The supernatant was collected and filtered using a Whatman No. 1 filter (qualitative filter paper, Advantec, Japan) and applied onto a nickel-agarose (Quiagen, Hilden, Germany) affinity column which had been pre-equilibrated with the binding buffer. The column was then washed with two column volumes of washing buffer which consisted of 50 mM phosphate pH 8.0, 500 mM NaCl, 5 mM -mercaptoethanol, 30 mM imidazole. The bound proteins were eluted with 50 mM Tris-HCl pH 8.0, 100 mM NaCl, 300 mM imidazole, 5 mM -mercaptoethanol.
After elution, fractions containing CtPGT were pooled and the protein solution was exchanged into buffer consisting of 50 mM Tris-HCl pH 8.0, 5 mM -mercaptoethanol by ultrafiltration (Centricon YM-30, Millipore Corporation, Bedford, Massachusetts, USA). To cleave the His tag from the protein (the His tag and TEV cleavage site are underlined in Table 1) the protein was treated with TEV protease (1:20 molar ratio for the protein sample) overnight at 277 K (Table 1). To separate the cleaved proteins, the reaction mixture was further loaded onto an Ni-NTA column. Flowthrough fractions containing CtPGT were concentrated and injected onto a Mono Q column (GE Healthcare, Piscataway, New Jersey, USA) equilibrated with buffer consisting of 50 mM Tris-HCl pH 8.0, 5 mM -mercaptoethanol. The protein was eluted using a salt gradient of 0-0.5 M NaCl in the same buffer using an FPLC system (GE Healthcare, Piscataway, New Jersey, USA). The peak fractions were collected and concentrated. Finally, pure CtPGT protein was separated by gel-filtration chromatography using a Superdex 200 column (GE Healthcare, Piscataway, New Jersey, USA) with buffer consisting of 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM DTT in an FPLC system. All purification steps were performed with icecooled buffers at room temperature, which we believe keep the protein stable. The protein purity was checked by SDS-PAGE and native PAGE, and its concentration was determined by the Bradford assay (Zor & Selinger, 1996;Bradford, 1976) using bovine serum albumin as a standard.  Table 1 Macromolecule-production information.

Crystallization
Initial crystallization of the selenium-labelled CtPGT was performed with the commercially available screening kits Crystal Screen, Crystal Screen 2 and Index from Hampton Research, California, USA and Wizard Classic 1 and 2 and Wizard Cryo 1 and 2 from Rigaku Reagents, Bainbridge Island, Washington, USA using the microbatch method under Al's oil in 72-well plates at 291 K. A crystallization drop consisted of 1 ml protein solution (10 mg ml À1 ) and 1 ml screening kit solution. Crystals appeared in 0.2 M triammonium citrate pH 7.0, 20%(w/v) PEG 3350. The hanging-drop vapour-diffusion method was performed to optimize this buffer condition in 24-well cell-culture plates by varying the concentrations of the buffer and precipitant around the condition that produced crystals. Finally, a single crystal that was large enough for diffraction ($0.4 mm) formed in 4 d using 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350. The CtPGT crystals were then soaked with 10 mM uridine-N-acetylglucosamine (UDP-NAG) and 5 mM dihydrobiopterin (l-erythro-BH 2 ) for complex preparation. CtPGT was also incubated with 10 mM uridine-N-acetylglucosamine (UDP-NAG) and 5 mM dihydrobiopterin (l-erythro-BH 2 ) in order to grow complex crystals by the co-crystallization method. Crystallization information is summarized in Table 2.     X-ray diffraction image from a CtPGT protein crystal. The black circle represents 2.14 Å resolution.

Data collection and processing
liquid nitrogen. Multiple-wavelength anomalous diffraction (MAD) data were collected from a single CtPGT crystal on beamline 7A at Pohang Accelerator Laboratory (PAL), Republic of Korea. 360 frames were collected with an oscillation angle of 1 and 1 s exposures with a crystal-to-detector distance of 280 mm. Three data sets were collected at peak (0.979184 Å ), edge (0.97934 Å ) and remote (0.971549 Å ) wavelengths at 100 K. All diffraction images were indexed, integrated and scaled using the HKL-2000 suite (Otwinowski & Minor, 1997). Data-collection details are shown in Table 3. Self-rotation function calculated for CtPGT crystal data, showing the = 180 and = 120 sections. Peaks are represented as dense contour lines. The calculation was performed using the diffraction data to 3 Å resolution.

Results and discussion
The gene for CtPGT from C. tepidum was successfully cloned in the pProEX HTa expression vector. This plasmid was transformed into E. coli BL21(DE3) cells for protein expression. Since CtPGT shares low sequence identity with other glycosyltransferases deposited in the PDB, direct phasing was attempted for structure determination. Therefore, selenomethionine-substituted protein was expressed from E. coli BL21(DE3) cells. The expressed protein was soluble and stable. The protein was purified sequentially by nickel-affinity, anion-exchange and gel-filtration chromatography. From the gel-filtration elution profile, the CtPGT protein was found to be a monomer in solution, with an estimated molecular weight of about 42 kDa, which is close to the value calculated from the number of amino acids (356 amino acids; Fig. 1a). Crystals of CtPGT were obtained in 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350 (Fig. 1c). MAD data sets were collected from a CtPGT crystal to 2.14 Å resolution (Fig. 2) (Matthews, 1968), with a solvent content of 57.89% for three chains, which suggests that there are three molecules in the asymmetric unit. The self-rotation function of the CtPGT crystal data from MOLREP (Winn et al., 2011) clearly showed two prominent peaks at = 180 , indicating that there are two noncrystallographic twofold symmetry axes between two molecules among the three molecules in the asymmetric unit. At = 120 no peaks were observed for a trimer (Fig. 3). We determined the CtPGT structure using the peak data by SAD phasing in PHENIX (Adams et al., 2010). MAD was not as successful as SAD. This seems to be because of crystal decay from continuous exposure to radiation, as seen for the data in the remote data set (Table 3). As a result of PHENIX SAD phasing, eight of the nine Se atoms in the molecule were found, which provided a starting electron-density map that was clear enough for model building after density modification. From the C chain trace, three molecules pack in the asymmetric unit and two pairs among these three molecules are associated by twofold noncrystallographic symmetry, as shown by the self-rotation function (Fig. 4). The detailed structure will be published soon.

Figure 4
CtPGT crystal packing. The C chain traces of three molecules are shown in different colours (A in blue, B in green and C in red) in the unit cell (red lines). Other molecules generated by crystal symmetry are displayed in grey.