X-ray crystallographic and high-speed AFM studies of peroxiredoxin 1 from Chlamydomonas reinhardtii

The oligomeric state of the 2-Cys peroxiredoxin from the green alga Chlamydomonas reinhardtii was determined by an X-ray crystallographic study and high-speed AFM image analysis.


Introduction
Photosynthesis is a light-dependent chemical process that converts light energy to chemical energy. Light energy is absorbed by chlorophylls or carotenoids in antenna proteins, and is then transferred to photosystem complexes, either photosystem II or I, which perform charge separation at the core of the complex. In nature, the intensity of light perceived by organisms can fluctuate extensively and sometimes exceeds the photosynthetic capacity of the organism. High-intensity light may lead to the production of reactive oxygen species (ROS) such as singlet oxygen ( 1 O 2 ), superoxide (O À 2 ), hydroxyl radicals (HO ) or hydrogen peroxide (H 2 O 2 ). These ROS are harmful to photosystems and other chloroplast proteins, and may induce photoinhibition (Takahashi & Badger, 2011). Plants and other organisms have developed several mechanisms to avoid light-induced photoinhibition, such as ROS-scavenging systems (Asada, 2006), phototaxis in green alga (Erickson et al., 2015) and nonphotochemical quenching, which converts the excess light into thermal energy that can be dissipated.
Peroxiredoxins (PRXs) are a group of antioxidant enzymes that are present in all organisms, including plants. In the green alga Chlamydomonas reinhardtii, PRXs are classified into four types based on the number and position of cysteine residues: ISSN 2053-230X 2-Cys PRX, 1-Cys PRX, PRX-Q and type II PRX (Dayer et al., 2008). The 2-Cys PRX from C. reinhardtii (CrPRX1) is a chloroplast-localized protein that is important for detoxifying ROS in the chloroplast. CrPRX1 can be activated by various types of thioredoxins (TRX; Sevilla et al., 2015) and its expression is regulated by light and oxygen concentration (Goyer et al., 2002).
In general, the oligomeric state of 2-Cys PRX is a dimer or a decamer, depending on the redox state, protein concentration, pH or its physiological function (Barranco-Medina et al., 2009). In the crystal structure, the human homologues of 2-Cys PRX, peroxiredoxin 3 (PRX3; PDB entry 5jcg) and peroxiredoxin 4 (PRX4; PDB entry 3tkp), form decamers (Yewdall et al., 2016;Wang et al., 2012). The cryo-TEM structure of PRX3 shows that decameric ring structures are stacked into a long helical filament, which might function as a self-associating chaperone. The crystal structure of 2-Cys PRX from the bacterium Salmonella typhimurium shows that the oxidized enzyme is in the decameric state, and its signalling activity is regulated by a redox-sensitive state change from a dimer to a decamer (Wood et al., 2002). Regarding plants, the crystal structure of PRX (type II) from Populus trichocapa has been reported (Echalier et al., 2005). This plant PRX forms a dimer in both the crystal (PDB entry 1tp9) and in solution, as confirmed by X-ray crystallography and NMR spectroscopy, respectively. Thus, the oligomerization of PRXs is functionally important, but is very difficult to predict based only on aminoacid sequence information.
Recently, we found that CrPRX1 is activated not only by TRXs but also by calredoxin (CrCRX), a novel chloroplastlocalized calcium-dependent protein consisting of calmodulin and thioredoxin domains (Hochmal et al., 2016). A possible mode of interaction between CrPRX1 and CrCRX was proposed on the basis of the crystal structure of CrCRX (PDB entry 5e37), but the preliminary discussion was limited owing to a lack of structural information on CrPRX1, especially on its oligomeric state. Because C. reinhardtii is a model organism in photosynthesis research, structural information on CrPRX1 would be useful for further analysis of photoacclimation in plant physiology or plant biology, as well as photosynthesis research, and is particularly important for the understanding of Ca 2+ -dependent electron transfer between CrPRX1 and CrCRX. The aim of the present study was therefore to analyze a single-particle image of recombinant CrPRX1 using highspeed atomic force microscopy (HS-AFM) and to crystallize CrPRX1 for further structural analysis. Based on the lowresolution HS-AFM image of CrPRX1 and the crystallographic analysis described here, we discuss the oligomeric state of CrPRX1.

Materials and methods
2.1. Cloning, protein expression and purification, and biochemical estimation of molecular weight The plasmid for the expression of recombinant C. reinhardtii peroxiredoxin 1 (CrPRX1) was constructed as described previously (Hochmal et al., 2016) and summarized in Table 1. The pET-22b CrPRX1 plasmid was transformed into Escherichia coli strain BL21(DE3). A single Cys174Ser (C2S) mutant of CrPRX1, which is a mutant that mimics the reduced active-site cysteine residues, was engineered in the pET-22b CrPRX1 plasmid by an In-Fusion Cloning kit (Takara Bio USA, Mountain View, California, USA) using the primers 5 0 -GAGGTCAGCCCCGCCGGCTGGAAG-3 0 and 5 0 -GGC GGGGCTGACCTCATCGGGGTT-3 0 . Wild-type and C2S mutant cells were grown in LB medium with 100 mg ml À1 ampicillin and incubated at 310 K with shaking until the OD 600 reached 0.5-0.6. Protein expression was induced with 0.5 mM isopropyl -d-1-thiogalactopyranoside, and the cells were further incubated at 310 K for 4-6 h. The cells were harvested by centrifugation at 8000g for 10 min and stored at 193 K. The cell pellets were suspended in lysis buffer consisting of 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM phenylmethylsulfonylfluoride and disrupted by sonication. The lysate was clarified by ultracentrifugation at 164 430g for 30 min and the supernatant was applied onto Ni-NTA resin (Qiagen, Hilden, Germany) pre-equilibrated with 50 mM Tris-HCl pH 8.0, 150 mM NaCl. The Ni-NTA resin was washed and eluted with 20 and 250 mM imidazole in equilibration buffer. The CrPRX1 protein was further purified using gel filtration on a Superdex 200 HR 16/60 column (GE Healthcare). The column was equilibrated with 20 mM HEPES pH 7.5, 150 mM NaCl. Purified protein fractions were concentrated by centrifugation using Amicon filter units with a membrane nominal molecularweight limit of 10 kDa (Merck Millipore, Cork, Ireland) for crystallization. Purified protein fractions were concentrated and kept at 193 K for crystallization (Fig. 1a).
The molecular weight of wild-type CrPRX1 was first estimated using a gel-filtration column with several protein markers. To verify the estimated molecular weight of CrPRX1 both in solution and from dissolved crystal samples, 20 mg protein sample or several crystals of CrPRX1 were loaded onto gels for SDS-PAGE and native PAGE (NativePAGE Novex 4-16% Bis-Tris Gels) analysis (Fig. 1). Macromoleculeproduction information is summarized in Table 1.

Crystallization
Crystallization conditions for CrPRX1 were screened by the hanging-drop vapour-diffusion method using Crystal Screen, Crystal Screen 2, PEG Rx 1, PEG Rx 2 (Hampton Research, Aliso Viejo, California, USA) and Wizard I, II, III and IV (Rigaku, Bainbridge Island, Washington, USA) at 277 and 293 K. Needle crystals of wild-type CrPRX1 were produced using 0.2 M ammonium acetate, 0.1 M sodium citrate pH 5.5, 24% PEG 400. After optimization including a microseeding technique, crystals with a suitable size for an X-ray experiment were obtained from droplets comprised of 1 ml of 30 mg ml À1 protein sample and 1 ml reservoir solution consisting of 0.05 M ammonium acetate, 0.1 M sodium citrate pH 5.5, 20% PEG 400, 15% glycerol (Fig. 2a). Unexpectedly, the crystallization conditions for the C2S mutant of CrPRX1 differed markedly from those for the wild type. After optimization of the crystallization conditions, rhombic crystals of the C2S mutant were obtained from droplets comprised of 2 ml of 15 mg ml À1 protein sample and 1 ml reservoir solution consisting of 0.1 M HEPES pH 7.6, 0.2 M sodium thiocyanate, 20% PEG 3350 (Fig. 2b). Crystallization information is summarized in Table 2.

X-ray data collection and processing
Single wild-type CrPRX1 crystals were picked up and directly cooled in liquid nitrogen. Single crystals of the C2S mutant were picked up and first soaked in 20% glycerol for cryoprotection before being cooled in liquid nitrogen. X-ray   diffraction images were collected from single crystals of wildtype and C2S mutant CrPRX1 on the BL44XU beamline at SPring-8. Data sets for wild-type CrPRX1 were collected at 100 K using a CCD-based MX300-HE detector system with an oscillation angle of 0.5 per frame, a crystal-to-detector distance of 450 mm and an exposure time of 1 s per frame. Data sets for C2S mutant CrPRX1 were collected at 100 K using the same CCD-based detector. The oscillation angle per frame was 0.3 and the crystal-to-detector distance was 350 mm. The exposure time per frame was 1 s. The diffraction data for the wild type were processed with the HKL-2000 software package (Otwinowski & Minor, 1997) and the data for the C2S mutant were processed with the XDS program package (Kabsch, 2010). Data-collection and processing statistics are summarized in Table 3.

High-speed atomic force microscopy (HS-AFM)
The concentration of CrPRX1 was 0.1 mg ml À1 in imaging buffer (50 mM sodium acetate pH 5.5, 150 mM NaCl). 2 ml of 100 mM CoCl 2 was loaded onto freshly cleaved mica substrate for 3 min at room temperature. After washing the mica surface with imaging buffer, 2 ml of CrPRX1 was loaded onto the mica surface and then incubated for 5 min at room temperature. After washing the mica surface with imaging buffer, HS-AFM imaging was performed in amplitude-modulation mode using a laboratory-built HS-AFM setup. The cantilever used was BL-AC10DS-A2 (Olympus Co., Tokyo, Japan) with a tip fabricated by electron-beam deposition.

Results and discussion
Recombinant CrPRX1 protein comprising 221 amino acids was successfully overexpressed and purified to electrophoretic homogeneity, showing a molecular weight of 24.7 kDa on SDS-PAGE (Fig. 1a). Native PAGE and gel-filtration chromatographic analysis showed that CrPRX1 exists as an oligomer in solution (Fig. 1b). The molecular mass of CrPRX1 from both dissolved crystals and purified solution was estimated to be about 350-480 kDa, indicating a clearly greater molecular mass than that of the homodimer, in contrast to the

Figure 3
Diffraction image of a CrPRX1 crystal of the C2S mutant recorded on BL44XU at SPring-8. oligomeric state of type II PRX1 from higher plants (Echalier et al., 2005). However, the solution sample eluted from gelfiltration column chromatography (lanes 4 and 5 in Fig. 1b) also contained a band at about 66 kDa corresponding to the dimeric form. This observation suggests that CrPRX1 can adopt both dimeric and higher oligomeric forms but prefers the oligomeric form, which can be crystallized. The crystal of wild-type CrPRX1 was found to belong to the hexagonal space group P6 3 , with unit-cell parameters a = b = 137.12, c = 354.87 Å , but only diffracted to 4.8 Å resolution (Table 2). In contrast, the crystal of the C2S mutant was found to belong to space group C222 1 , with unit-cell parameters a = 134.81, b = 418.11, c = 94.32 Å , and diffracted to 2.4 Å resolution, thus being suitable for atomic structure analysis (Fig. 3). This improvement in crystal quality may be owing to the different packing of the C2S mutant crystals. For phasing, we carried out molecular replacement using the program Phaser MR in the CCP4 program suite (Collaborative Computational Project, Number 4, 1994;Winn et al., 2011). The best homologous model for the molecularreplacement calculations was thioredoxin peroxidase B from human red blood cells (PDB entry 1qmv;Schrö der et al., 2000), with 63% sequence identity to CrPRX1. Structure determination, including model rebuilding and refinement, is  now in progress, but we note that the self-rotation functions calculated using the diffraction data from the C2S crystal show an obvious peak in the = 72 section corresponding to the existence of noncrystallographic fivefold symmetry (Fig. 4).
Importantly, HS-AFM images revealed that the wild-type CrPRX1 particles formed rings with pentagonal rotational symmetry in solution (Fig. 5). This corresponds well to the fivefold symmetry found in the self-rotation function described above, illustrating that the noncrystallographic fivefold symmetry is intramolecular. The average peak height of AFM images from the mica surface was 2.7 AE 0.3 nm (n = 30). The observed outer diameter of the CrPRX1 ring was 16.8 AE 0.94 nm (n = 30) on average. Together with the observed fivefold symmetry and the experimentally measured height and diameter of CrPRX1, the possibility of a highly stacked ring structure, as found in the filamentous oligomeric state of PRX3 (Yewdall et al., 2016), is unlikely. Although the only available structure of a plant PRX (that from P. trichocapa) is homodimeric (PDB entry 2pwj; Barranco-Medina et al., 2006), the search model for molecular replacement with the highest sequence identity (PDB entry 1qmv) possessed a decameric ring structure. Considering other examples of 2-Cys PRXs, such as PRX3, PRX4 and PRX5, we conclude that the oligomeric state of CrPRX1 is a ring-shaped decamer formed by a pentamer of dimers both in the crystal and solution states. Efforts towards structure determination using the molecularreplacement method are currently progressing well, and will provide a clearer insight into the oligomeric state of CrPRX1, as well as being essential for elucidating the functional interaction with TRXs and CrCRX.