Figure 3
NS-TEM images of lipid–detergent phases formed in protein-free control samples and crystallization drops. For reference, the samples are plotted on the crystallization phase diagrams on the right. (a) A protein-free control sample with l/d ratio corresponding to the precipitation zone of MhsT, showing modular multilamellar liposomal structure. (b) Similar to (a), but with p/l/d ratio corresponding to the crystallization zone of MhsT. (c), (d) An MhsT sample from the precipitation zone showing putative cylindrical micelles. (e) An MhsT sample from the crystallization zone showing liposomes. (f) An MhsT sample from the crystallization zone showing small spherical particles and/or discs. (g) An MhsT sample from the crystallization zone showing structures with a tangled worm-like appearance. (h) A SERCA1a crystallization drop showing liposomes. (i) A SERCA1a crystallization drop showing fingerprint-like putative micelles. The scale bars are 100 nm (white) or 50 nm (black). |