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Carbamoyl Phosphate synthetase catalyzes the formation of carbamoyl phosphate, a high-energy intermediate used in several biosynthetic pathways. The enzyme from Escherichia coli has been crystallized at pH 8 in the presence of L-ornithine, MnCl2 and ADP, using PEG 8000 in combination with NEt4Cl and KCl. The crystals (apparently) belong to the orthorhombic space group P212121 with unit-cell dimensions of a = 154.4, b = 166.5 and c = 338.7 Å. The crystals are relatively sensitive to radiation damage, but show diffraction to beyond 2.8 Å resolution. A low-resolution (3.5 Å) native data set has been recorded and conditions for flash cooling the crystal have been established.
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