Purification, crystallization and preliminary X-ray diffraction of anthocyanidin synthase from Arabidopsis thaliana

Anthocyanidin synthase (ANS) from Arabidopsis thaliana is a non-haem iron(II)-dependent dioxygenase reported to catalyse the conversion of leucoanthocyanidins to anthocyanidins. Anthocyanidins are precursors of anthocyanins, which are a major family of pigments in higher plants. ANS was crystallized by the vapour-diffusion method using polyethylene glycol as a precipitant. The crystals belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 61.0, b = 73.2, c = 87.0 Å, and diffract to 2.4 Å using Cu Kα radiation.