Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum

Lu, C.-T.; Tada, T.; Nakamura, Y.; Wada, K.; Nishimura, K.; Katsuya, Y.; Sawada, M.; Takao, M.; Sakai, T.

doi:10.1107/S0907444900013305

Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum

C.-T. Lu, T. Tada, Y. Nakamura, K. Wada, K. Nishimura, Y. Katsuya, M. Sawada, M. Takao and T. Sakai

Endopolygalacturonase SE1 from Trichosporon penicillatum has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as a precipitant. The crystals belong to the hexagonal space group P6₁ or P6₅, with unit-cell parameters a = b = 135.0, c = 70.7 Å, γ = 120°. The calculated V_M based on one molecule per asymmetric unit is 3.09 Å³ Da⁻¹. A native data set from a crystal has been collected to 2.0 Å resolution on a Cu Kα rotating-anode X-ray source.

Keywords: endopolygalactouronase SE1; pectolytic enzymes.

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Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum

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