Crystallization and preliminary X-ray analysis of human liver α-enolase

Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. α-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 Å resolution. The crystals belonged to space group P2₁, with unit-cell parameters a = 72.85, b = 66.02, c = 79.43 Å, β = 94.54°.