Antigen recognition by antibody C836 through adjustment of VL/VH packing

Teplyakov, A.; Obmolova, G.; Malia, T.; Gilliland, G.

doi:10.1107/S1744309111027746

Antigen recognition by antibody C836 through adjustment of V_L/V_H packing

A. Teplyakov, G. Obmolova, T. Malia and G. Gilliland

C836 is a neutralizing monoclonal antibody to human interleukin IL-13 generated by mouse immunization. The crystal structure of the C836 Fab was determined at 2.5 Å resolution and compared with the IL-13-bound form determined previously. This comparison indicates an induced-fit mechanism of antigen recognition through rigid-body rotation of the V_L and V_H domains. The magnitude of this rearrangement is one of the largest observed for antibody-protein interactions.

Keywords: monoclonal antibodies; induced fit; V_L/V_H packing.

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Antigen recognition by antibody C836 through adjustment of V_L/V_H packing

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