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The native SAD phasing method uses the anomalous scattering signals from the S atoms contained in most proteins, the P atoms in nucleic acids or other light atoms derived from the solution used for crystallization. These signals are very weak and careful data collection is required, which makes this method very difficult. One way to enhance the anomalous signal is to use long-wavelength X-rays; however, these wavelengths are more strongly absorbed by the materials in the pathway. Therefore, a crystal-mounting platform for native SAD data collection that removes solution around the crystals has been developed. This platform includes a novel solution-free mounting tool and an automatic robot, which extracts the surrounding solution, flash-cools the crystal and inserts the loop into a UniPuck cassette for use in the synchrotron. Eight protein structures (including two new structures) have been successfully solved by the native SAD method from crystals prepared using this platform.

Supporting information

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Portable Document Format (PDF) file https://doi.org/10.1107/S2059798320011584/yl5005sup1.pdf
Supplementary Table S1 and caption for Supplementary Movie S1.

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Moving Picture Experts Group (MP4) video file https://doi.org/10.1107/S2059798320011584/yl5005sup2.mp4
Supplementary Movie S1.


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