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Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P21, with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Å, β = 103.86°, and diffract to 1.5 Å resolution.

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