crystallization communications
Ca2+-ATPases are ATP-driven membrane pumps that are responsible for the transport of Ca2+ ions across the membrane. The Listeria monocytogenes Ca2+-ATPase LMCA1 has been crystallized in the Ca2+-free state stabilized by AlF4-, representing an occluded E2-Pi-like state. The crystals belonged to space group P21212 and a complete data set extending to 4.3 Å resolution was collected. A molecular-replacement solution was obtained, revealing type I packing of the molecules in the crystal. Unbiased electron-density features were observed for AlF4- and for shifts of the helices, which were indicative of a reliable structure determination.