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Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

Supporting information

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Portable Network Graphics (PNG) image https://doi.org/10.1107/S2053230X15002265/nw5017sup1.png
Figure S1. Anomalous difference Patterson map of the section z = 0.5 at 3.5 Å resolution. Contour levels from 3σ to 10σ are drawn in a 0.5σ steps.

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Portable Network Graphics (PNG) image https://doi.org/10.1107/S2053230X15002265/nw5017sup2.png
Figure S.2 An electron-density map by SAD phasing using the program autoSHARP. The electron density was contoured at the level of 1σ. A polyalanine model from AutoSHARP is also shown.


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