Crystallization and preliminary X-ray study of endopolygalacturonase from the pathogenic fungus Stereum purpureum

Crystals of endopolygalacturonase I from Stereum purpureum have been obtained by the vapour-diffusion method. Prior to crystallization work, endopolygalacturonase I was deglycosylated with endo-β-N-acetylglucosaminidase H. The crystal diffracts to ultrahigh (0.96 Å) resolution using synchrotron radiation and belongs to space group P1, with unit-cell parameters a = 37.26, b = 46.34, c = 52.05 Å, α = 67.17, β = 72.44, γ = 68.90°.