Crystallization and preliminary X-ray crystallographic studies of the sixth PDZ domain of glutamate-receptor interacting protein 1 (GRIP1) from Rattus norvegicus

The sixth PDZ domain from GRIP1 and its complex with the octapeptide of the liprin-α1 C-terminus were crystallized at 294 K by the hanging-drop vapour-diffusion method. The native crystal belongs to space group P6₁22 (or P6₅22), with unit-cell parameters a = b = 40.3, c = 222.9 Å. The complex crystal belongs to space group R32, with unit-cell parameters a = b = 117.8, c = 102.0 Å. Native and peptide-complex diffraction data were collected to resolutions of 1.5 and 1.8 Å, respectively, using synchrotron X-rays.