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 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
CCP-EM Spring Symposium 2024
Edited by Tom Burnley, Paula da Fonseca, Peter Rosenthal and Peijun Zhang
This virtual issue collects together articles from the 2024 CCP-EM Spring Symposium.
Cover illustration: Recent studies demonstrate that the range of samples suitable for cryo-EM single-particle analysis (SPA) is expanding towards increasingly more native samples. Tailored sample-preparation strategies make complex macromolecular assemblies amenable for SPA, while still keeping them in close-to-native conditions. The 96 nm repeat of axonemal doublet microtubules showing all the external complexes is shown here as an example.
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This article reviews the growth over the last ten years of the CCP-EM Spring Symposium. The conference is now established as a popular meeting for practitioners of life-science cryoEM and highlights new methods and exemplar applications.
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Cryo-EM single-particle analysis has revolutionized structural biology by allowing high-resolution analysis of large molecular assemblies that are not amenable to crystallography or NMR. Combination of this technology with innovative sample-preparation protocols further expands the range of potential targets.
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We introduce a new approach to probabilistic multi-volume single-particle cryo-EM ab initio 3D reconstruction for simultaneous estimation of the relative particle 3D orientations and partitioning of the particles into groups with distinct structural states.
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Q-scores are calculated for atomic models derived from 3D electron microscopy maps, measure how well the model fits the map and reflect the quality of the map itself. Here, we develop a statistical model for Q-scores applied to many maps and models in the EMDB and PDB, respectively, and show how it can be used to assess the reliability of entire models as well as their subcomponents.
