The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases
aLaboratory of Non-coding RNA, Institute of Biophysics, Chinese Academy of Sciences, 5 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China,bPhotosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Haidian District, Beijing 100093, People's Republic of China, and cUniversity of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, People's Republic of China
*Correspondence e-mail: firstname.lastname@example.org, email@example.com
Plant Deg5 and Deg8 are two members of the HtrA proteases, a family of oligomeric serine endopeptidases that are involved in a variety of protein quality-control processes. These two HtrA proteases are located in the thylakoid lumen and participate in high-light stress responses by collaborating with other chloroplast proteins. Deg5 and Deg8 degrade photodamaged D1 protein of the photosystem II reaction centre, allowing its in situ replacement. Here, the crystal structures of Arabidopsis thaliana Deg5 (S266A) and Deg8 (S292A) are reported at 2.6 and 2.0 Å resolution, respectively. The Deg5 trimer contains two calcium ions in a central channel, suggesting a link between photodamage control and calcium ions in chloroplasts. Previous structures of HtrA proteases have indicated that their regulation usually requires C-terminal PDZ domain(s). Deg5 is unique in that it contains no PDZ domain and the trimeric structure of Deg5 (S266A) reveals a novel catalytic triad conformation. A similar triad conformation is observed in the hexameric structure of the single PDZ-domain-containing Deg8 (S292A). These findings suggest a novel activation mechanism for plant HtrA proteases and provide structural clues to their function in light-stress response.
HtrA (high-temperature requirement A) proteases are a family of ATP-independent trypsin-like serine endopeptidases that belong to MEROPS subfamily S1C and are involved in protein quality control (PQC) in both prokaryotes and eukaryotes (Rawlings et al., 2008; Clausen et al., 2002; Ehrmann & Clausen, 2004). They have an N-terminal protease domain and 0–3 PDZ (PSD95/Dlg1/ZO-1) domain(s) at the C-terminal end. The functional unit of the HtrA proteases is a trimer, with three protease domains forming a central core. The physiological importance of the HtrA proteases can be inferred from their wide distribution from bacteria to humans. Three HtrA proteases from Escherichia coli, DegS, DegP and DegQ, are the best-characterized prokaryotic members (Singh et al., 2011). They are located in the periplasmic space and are involved in essential PQC processes. Human HtrA1 and HtrA2 are the most thoroughly studied eukaryotic members and both have been discovered to play key roles in development and disease (Clausen et al., 2011).
Their lack of motility renders plants constantly susceptible to environmental stresses. To deal with photodamage to their photosynthetic machinery, plants have evolved a sophisticated PQC system (Sakamoto, 2006; Eberhard et al., 2008). The members of the HtrA proteases have been identified as critical components of the chloroplast PQC system (Schuhmann et al., 2012; Komenda et al., 2012). Among the 16 Arabidopsis HtrA proteases that have been annotated, Deg1, Deg5 and Deg8 are in the chloroplast thylakoid lumen (Schuhmann & Adamska, 2012). Deg1 has been reported to be involved in photoinhibition repair and photosystem II (PSII) assembly through its dual protease and chaperone activity (Kapri-Pardes et al., 2007; Sun, Ouyang et al., 2010). Deg5 and Deg8 are required for efficient PSII repair under light stress and recombinant Deg8 is proteolytically active with β-casein as the substrate, whereas Deg5 demonstrates no β-casein degradation activity (Sun et al., 2007; Kato et al., 2012). Deg1, Deg5 and Deg8 together with two stromal HtrA members, Deg2 and Deg7 (Haussühl et al., 2001; Sun, Fu et al., 2010), participate in degradation of damaged reaction-centre protein D1 in the PSII complex during light stress.
The structures of members of the HtrA proteases from E. coli (DegS, DegP and DegQ) and of human HtrA1 and HtrA2 have provided detailed insights into their activation mechanisms. Trimeric DegS requires the binding of stress-signalling peptides to its PDZ domain for allosteric activation (Wilken et al., 2004; Sohn et al., 2007), while DegP and DegQ form large oligomers with encapsulated substrate (Krojer et al., 2008; Kim et al., 2011; Malet et al., 2012). Unlike DegS, the HtrA1 trimer and the HtrA2 trimer can degrade substrates directly without any other cofactors (Truebestein et al., 2011; Li et al., 2002).
Recent structural studies on Arabidopsis Deg1 have suggested a pH-dependent activation pathway (Kley et al., 2011). It has been shown that loops LA, L1, L2, L3 and LD play direct roles in the regulation of Deg1 activity. At acidic pH, Deg1 trimers dimerize through interactions between the PDZ domain and loop LA of the protease domain, leading to a fixed position of the PDZ domain and establishing a PDZ–L3–LD interaction network. As it is naturally devoid of a PDZ domain, Deg5 should employ a different activation mechanism. Although Deg8 is the closest homologue of Deg1 in the Arabidopsis genome (Schuhmann & Adamska, 2012), these two enzymes are functionally different. Deg1 is constitutively expressed and is required for plant growth, while Deg8 is sensitive to high light levels and together with Deg5 does not contribute significantly to the degradation of PSII D1 under low-light conditions (Kato et al., 2012). In addition, Deg5 and Deg8 have a synergistic effect on PSII D1 degradation during high light stress (Sun et al., 2007). All of these facts indicate that Deg5 and Deg8 utilize different mechanisms to regulate protease activity and the structural basis behind them needs to be examined.
Here, we report the crystal structures of Deg5 (S266A) and Deg8 (S292A) at 2.6 and 2.0 Å resolution, respectively. Both structures represent proteolytically incompetent states. The catalytic triad in both structures is malformed by rotation of the conserved histidine. We also find that the Deg5 trimer can bind two calcium ions. These findings provide novel structural evidence of the differing activities and regulation mechanisms of HtrA proteases.
The expression, purification, crystallization and preliminary diffraction analysis of Deg5 (S266A) and Deg8 (S292A) have been described previously (Fan et al., 2012; Shan et al., 2013).
All data collections were performed on beamline BL17U at Shanghai Synchrotron Radiation Facility (SSRF) using a MAR 225 CCD detector (MAR Research). Data were processed with HKL-2000 (Otwinowski & Minor, 1997).
Deg5 crystallized in space group C2, with three molecules per asymmetric unit that are related by a noncrystallographic symmetry threefold axis. The structure of Deg5 (S266A) was solved by the molecular-replacement method using MOLREP (Vagin & Teplyakov, 2010) within the CCP4 suite (Winn et al., 2011). HtrA from Thermotoga maritima (PDB entry 1l1j ; Kim et al., 2003) was used as the search model. The initial model was built with ARP/wARP (Cohen et al., 2008) and amino acids were then added manually in Coot (Emsley et al., 2010). Refinement was performed with REFMAC5 (Murshudov et al., 2011). Noncrystallography symmetry restraints were used. The final R and Rfree were 18.2% and 23.1%, respectively.
Deg8 (S292A) crystals were soaked in cryoprotectant (12% 2-methyl-2,4-pentanediol) and data collection was performed at a wavelength of 0.9792 Å in a nitrogen stream at 100 K. The Deg8 crystal belonged to space group C2 and one asymmetric unit contained three Deg8 molecules, which are also related by a noncrystallographic symmetry threefold axis. The Deg8 (S292A) structure was also solved by molecular replacement and the search model for Deg8 (S292A) was the structure of Arabidopsis Deg1 (PDB entry 3qo6 ; Kley et al., 2011), including both the protease and PDZ domains. The model was refined with PHENIX (Adams et al., 2010) and CNS (Brünger et al., 1998) and was built using Coot (Emsley et al., 2010) based on the Fo − Fc electron-density map. Further refinement was performed with REFMAC5 (Murshudov et al., 2011). The final R and Rfree were 19.8% and 23.5%, respectively.
The final models of Deg5 (S266A) and Deg8 (S292A) were validated with PROCHECK (Laskowski et al., 1993). Statistics of data collection and refinement are summarized in Table 1.
2.3. Inductively coupled plasma mass spectrometry (ICP-MS)
Analysis of calcium content was carried out using ICP-MS (Thermo Scientific) at the Tsinghua University Analysis Center (Beijing, People's Republic of China). Purified Deg5 (S266A) was dialyzed thoroughly against buffer consisting of 20 mM Tris–HCl pH 8.0, 150 mM NaCl before ICP-MS. To obtain calcium-stripped samples, 5 mM EDTA was added to the dialysis buffer. The ICP-MS data are summarized in Table 2.
10 µM protein was buffered in sodium phosphate pH 6.0 or 8.0, 150 mM NaCl and was incubated on ice for at least 1 h. Samples were then loaded onto a Superdex 200 10/300 GL (GE Healthcare) size-exclusion column and their molecular weights were calculated using a calibration curve determined using calibration standards (GE Healthcare).
For each assay, 100 µg β-casein was incubated in 200 µl 50 mM sodium phosphate pH 6.0, 50 mM NaCl with or without 20 µg recombinant Deg8 or 2 µg recombinant Deg1 at 310 K. The reaction mixtures at different time points were subjected to SDS–PAGE on a 12% acrylamide gel.
His147, Asp188 and Ser266 constitute the catalytic triad of Deg5. To avoid self-degradation of wild-type Deg5 during crystallization, Ser266 was mutated to alanine (Fan et al., 2012). The crystal of Deg5 (S266A) belonged to space group C2 and contains a trimer with a threefold noncrystallographic axis of symmetry in the asymmetric unit (Fig. 1a). The trimer has a shallow funnel shape and the catalytic triad is located on the concave side of the funnel. Five Ca2+ ions are observed per trimer. Three are on the convex side and each interacts with the N-terminal acidic residues of each protomer. The other two are located on the noncrystallographic threefold axis, along which a channel is formed (Figs. 1a and 1b). In the crystal two Deg5 (S266A) trimers associate with each other through their convex surfaces. In the back-to-back hexamer the interactions are mainly hydrogen bonds and salt bridges involving the Ca2+ ions on the surfaces. The molecular weight of Deg5 (S266A) as measured by size-exclusion chromatography (SEC) and dynamic light scattering is approximately 100 kDa, indicating that the trimer is the solution species and that two trimers only associate in the crystal.
Trimerization of the protomers is mediated through hydrophobic interactions, hydrogen bonds and a π–cation interaction. A hydrophobic interface is formed by residues of two regions. One includes Leu231 and Ile233 on β9, Phe95 on the N-terminal helix α1, Val235* on β9 (where the asterisk denotes the participation of the neighbouring protomer) and Val214* on the loop before β8; the other includes Tyr225 on loop LD and Val296* and Phe298* on β13 (Supplementary Fig. S1a1). Hydrogen-bond interactions are found in the area around the hydrophobic interface and include the following pairs: Glu83–Tyr274*, Glu85–Arg90*, Glu88–Ser217*, Glu88–Ser273*, Asn91–Gly215*, Gln96–Arg213*, Leu231–Ser237* and Ile233–Asp258* (Supplementary Fig. S1b). Moreover, Tyr227 donates π-electrons to the H atom on the N∊ atom of Arg241*, which also contributes to trimer stabilization (Supplementary Fig. S1c).
The electrostatic surface of the central channel in the Deg5 (S266A) trimer is highly acidic (Fig. 1b, top and left). The carboxyl groups of Glu87 and Asp260 stabilize two Ca2+ ions at both ends of the channel (Fig. 1b, bottom). ICP-MS analysis of the Deg5 (S266A) solution sample quantified that the Ca2+:protomer ratio was 0.60, corresponding to 1.8 Ca2+ ions per trimer (Table 2). In the crystal there are five Ca2+ ions. The three excess Ca2+ ions bound to the N-terminal acidic residues of Deg5 (S266A) (Fig. 1b, top and right) were probably introduced during crystallization. To test whether the central two Ca2+ ions coordinated by acidic residues (Glu87 and Asp260) are required for Deg5 trimerization, we mutated both Glu87 and Asp260 to alanine. SEC revealed that the mutant (E87A/D260A/S266A) exists predominantly as a trimer (Fig. 1c). ICP-MS analysis of this triple mutant quantified that the Ca2+:protomer ratio was 0.12, corresponding to 0.36 Ca2+ ions per trimer. These results suggested that the two Ca2+ ions observed in the crystal structure are also accommodated by the two acidic residues in solution.
Deg5 may act as Ca2+-storage protein in chloroplasts. To test this possibility, Deg5 (S266A) was dialyzed against 5 mM EDTA and the Ca2+:protomer ratio was analyzed by ICP-MS (Table 2). The ratio decreased to 0.037 Ca2+ ions per protomer and analysis of the oligomeric state using SEC confirmed that Deg5 remained as a trimer (Fig. 1c). These results indicated that the Ca2+ ions can be readily stripped by EDTA and are dispensable for Deg5 trimerization, indicating a potential role of Deg5 in calcium signalling. Calcium signals are critical for plant development and stress adaptation (Kudla et al., 2010). A recent publication proposed that the thylakoid luminal calcium concentration is a signalling mechanism for PSII oxygen-evolving complex assembly and D1 repair (Lohmiller et al., 2012). The Deg5 (S266A) structure reported here thus provides new clues to the function of Deg5 in PSII quality control. Given the importance of chloroplasts in calcium signalling (Stael et al., 2012), the biological function of Ca2+ ions in the Deg5 central channel awaits further studies.
In the Deg5 (S266A) trimer, the side chain of the catalytic His147 has two conformations (Figs. 2a and 2b). One contains the conserved hydrogen bond between His147 and Asp188, while the alternative conformation differs from that in any reported HtrA structure (Clausen et al., 2011). The χ1 angle of His147 in the alternative conformation is rotated anticlockwise by ∼120°, preventing formation of the His147–Asp188 hydrogen bond, which is an unusual conformation compared with other distorted HtrA catalytic triads (Krojer et al., 2002; Kim et al., 2003; Wrase et al., 2011). It resembles the catalytic histidine of human HtrA1 (Truebestein et al., 2011; Eigenbrot et al., 2012), but in that case the imidazole ring is rotated clockwise around the χ1 axis (Fig. 2c).
In Deg5 (S266A), residues 111–125 from loop LA, 155–159 from loop LB and 287–295 from loop L2 cannot be traced in the electron-density map. These loops, together with loop L3 (241–251), which has significantly high B-factor values, are flexible in the crystal. Loops L1, L2 and LD are defined as the activation domain, the proper conformation of which indicates activated HtrA proteases (Krojer et al., 2002, 2010; Kley et al., 2011), with L1 being linked to oxyanion-hole formation, L2 to the substrate-specificity pocket and LD being defined as the activation loop. Thr284 of loop L2 stretches close to the active site and forms a hydrogen bond to His147 (Fig. 2a) which blocks substrate from approaching the catalytic triad. The missing electron density for loops including L2, together with the distorted His147, indicates that the Deg5 structure shown here is of an inactive form. Deg5 may become proteolytically active after the conformation of loops L1 and L2 is changed by allosteric or substrate activation.
The crystallized Deg8 (S292A) contains 358 amino-acid residues (Leu91–Ser448), of which residues 104–448 could be defined in the electron-density map. Deg8 (S292A) forms a hexamer in the crystals (Figs. 3a and 3b). Interestingly, when superimposing one trimer on Deg1 or DegP6 (DegP hexamer), the orientation of the opposite trimer of the Deg8 protease domains differs from that of Deg1 but is more similar to that of DegP6 (Fig. 3a). Despite the higher similarity between Deg1 and Deg8, when compared with Deg1 (Kley et al., 2011) the orientation of the Deg8 PDZ domain changes by ∼35° along the major inertia axis (Fig. 3c). As such, the Deg8 PDZ domain is flattened out from the trimeric core made by the protease domain and the concavity of the trimeric funnel is shallower than in Deg1. The internal cavity of Deg8 (S292A) is smaller than that of the inactive DegP hexamer (Krojer et al., 2002; Fig. 3b), with their volumes being 46 143 and 69 527 Å3, respectively, as calculated by 3V (Voss & Gerstein, 2010). Such a compact hexameric assembly would prevent the substrate from accessing the active site.
Trimerization is primarily mediated by hydrophobic interactions and hydrogen bonds. The residues involved in hydrophobic interactions are Val240, Val261, Phe123*, Leu257*, Pro355, Phe251* and Phe253* (Supplementary Fig. S2a). Seven pairs of hydrogen bonds are found between two neighbouring protomers: Val232–Phe107*, Ser299–Glu116*, Gln243–Glu116*, Asp284–Val259*, Ser263–Leu257*, Ala318–Asn288* and Arg267–Asp254* (Supplementary Fig. S2b). Six pairs of hydrogen bonds are observed between Arg179 and Phe389* from opposite trimers, stabilizing the hexamer (Supplementary Fig. S2c). To test the oligomeric state of Deg8 in solution, we performed an analytical SEC assay (Fig. 3d). The SEC assay showed that Deg8 exists as trimers and monomers at pH 6.0. However, a small fraction of hexamer appeared at pH 8.0, indicating that Deg8 tends to form higher oligomers at basic pH rather than acidic pH (Fig. 3d). This behaviour differs from that of Deg1, the hexameric form of which is assembled at low pH (Kley et al., 2011), and from that of Deg2, the hexameric form of which is pH-independent (Sun et al., 2012).
The catalytic triad of Deg8 consists of His171, Asp214 and Ser292. In the Deg8 (S292A) structure, however, the triad fails to form catalytically competent hydrogen bonds owing to the anticlockwise rotation of the χ1 angle of His171 by ∼120° (Figs. 4a and 4b), which is similar to that of Deg5 (S266A) and differs from that of HtrA1 (Truebestein et al., 2011; Eigenbrot et al., 2012; Fig. 4a). His171 can form a hydrogen bond to Gln272 of loop L3, a unique feature that was not previously observed in HtrA proteases (Figs. 4a and 4b). Moreover, the orientation of loop L2 is quite different from those of other HtrA proteases such as Deg1 (Kley et al., 2011), the DegP 24-mer (Krojer et al., 2008), Deg5 and HtrA1 (Truebestein et al., 2011; Eigenbrot et al., 2012; Fig. 4c). Furthermore, we performed an activity assay on Deg8 using casein as the substrate. The recombinant Deg8 exhibits a remarkably lower proteolytic activity than that of Deg1 in degrading substrate (Supplementary Fig. S2d). This incompetent state suggests that to activate Deg8 a new activation mechanism may be employed for rearrangement of the activation domain.
Deg5 is a distinct HtrA protease that is naturally devoid of a PDZ domain. Protease activity has not been detected either in vivo (Sun et al., 2007) or in vitro. As revealed by the Deg5 (S266A) structure and ICP-MS analysis, trimeric Deg5 can bind two Ca2+ ions, suggesting a role in calcium events in chloroplasts. Deg5 (S266A) shows an inactive state in which a novel conformation of the catalytic His147 was observed and Thr284 on loop L2 interacts with His147 and blocks substrate entry. Deg8 contains one PDZ domain that shows a different orientation compared with that in the Deg1 hexamer. The Deg8 (S292A) hexamer has a narrower cavity and a distorted His171 like His147 in Deg5. Our observation of dramatically different conformations of the catalytic histidine reveals motion of this χ1 angle and provides new structural insights into the regulation mechanism of chloroplast HtrA proteases.
We thank the Shanghai Synchrotron Radiation Facility beamline scientists for technical support during data collection. This work was supported by the Ministry of Science and Technology of China (grants 2011CB910503, 2011CBA00901 and 2012CB917302) and the National Natural Science Foundation of China (grants 31021062 and 31170688).
Adams, P. D. et al. (2010). Acta Cryst. D66, 213–221. Web of Science CrossRef CAS IUCr Journals
Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J.-S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T. & Warren, G. L. (1998). Acta Cryst. D54, 905–921. Web of Science CrossRef IUCr Journals
Clausen, T., Kaiser, M., Huber, R. & Ehrmann, M. (2011). Nature Rev. Mol. Cell Biol. 12, 152–162. Web of Science CrossRef CAS
Clausen, T., Southan, C. & Ehrmann, M. (2002). Mol. Cell, 10, 443–455. Web of Science CrossRef PubMed CAS
Cohen, S. X., Ben Jelloul, M., Long, F., Vagin, A., Knipscheer, P., Lebbink, J., Sixma, T. K., Lamzin, V. S., Murshudov, G. N. & Perrakis, A. (2008). Acta Cryst. D64, 49–60. Web of Science CrossRef CAS IUCr Journals
Eberhard, S., Finazzi, G. & Wollman, F. A. (2008). Annu. Rev. Genet. 42, 463–515. Web of Science CrossRef PubMed CAS
Ehrmann, M. & Clausen, T. (2004). Annu. Rev. Genet. 38, 709–724. Web of Science CrossRef PubMed CAS
Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., Quan, C., Tom, J., Sandoval, W., van Lookeren Campagne, M. & Kirchhofer, D. (2012). Structure, 20, 1040–1050. Web of Science CrossRef CAS PubMed
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486–501. Web of Science CrossRef CAS IUCr Journals
Fan, H., Sun, W., Sun, Z., Gao, F. & Gong, W. (2012). Acta Cryst. F68, 839–841. Web of Science CrossRef IUCr Journals
Haussühl, K., Andersson, B. & Adamska, I. (2001). EMBO J. 20, 713–722. Web of Science PubMed
Kapri-Pardes, E., Naveh, L. & Adam, Z. (2007). Plant Cell, 19, 1039–1047. Web of Science PubMed CAS
Kato, Y., Sun, X., Zhang, L. & Sakamoto, W. (2012). Plant Physiol. 159, 1428–1439. Web of Science CrossRef CAS PubMed
Kim, S., Grant, R. A. & Sauer, R. T. (2011). Cell, 145, 67–78. Web of Science CrossRef CAS PubMed
Kim, D. Y., Kim, D. R., Ha, S. C., Lokanath, N. K., Lee, C. J., Hwang, H.-Y. & Kim, K. K. (2003). J. Biol. Chem. 278, 6543–6551. Web of Science CrossRef PubMed CAS
Kley, J., Schmidt, B., Boyanov, B., Stolt-Bergner, P. C., Kirk, R., Ehrmann, M., Knopf, R. R., Naveh, L., Adam, Z. & Clausen, T. (2011). Nature Struct. Mol. Biol. 18, 728–731. Web of Science CrossRef CAS
Komenda, J., Sobotka, R. & Nixon, P. J. (2012). Curr. Opin. Plant Biol. 15, 245–251. Web of Science CrossRef CAS PubMed
Krojer, T., Garrido-Franco, M., Huber, R., Ehrmann, M. & Clausen, T. (2002). Nature (London), 416, 455–459. Web of Science CrossRef PubMed CAS
Krojer, T., Sawa, J., Huber, R. & Clausen, T. (2010). Nature Struct. Mol. Biol. 17, 844–852. Web of Science CrossRef CAS
Krojer, T., Sawa, J., Schäfer, E., Saibil, H. R., Ehrmann, M. & Clausen, T. (2008). Nature (London), 453, 885–890. Web of Science CrossRef PubMed CAS
Kudla, J., Batistic, O. & Hashimoto, K. (2010). Plant Cell, 22, 541–563. Web of Science CrossRef CAS PubMed
Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. (1993). J. Appl. Cryst. 26, 283–291. CrossRef CAS Web of Science IUCr Journals
Li, W., Srinivasula, S. M., Chai, J., Li, P., Wu, J.-W., Zhang, Z., Alnemri, E. S. & Shi, Y. (2002). Nature Struct. Biol. 9, 436–441. Web of Science CrossRef PubMed CAS
Lohmiller, T., Cox, N., Su, J.-H., Messinger, J. & Lubitz, W. (2012). J. Biol. Chem. 287, 24721–24733. Web of Science CrossRef CAS PubMed
Malet, H., Canellas, F., Sawa, J., Yan, J., Thalassinos, K., Ehrmann, M., Clausen, T. & Saibil, H. R. (2012). Nature Struct. Mol. Biol. 19, 152–157. Web of Science CrossRef CAS
Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Long, F. & Vagin, A. A. (2011). Acta Cryst. D67, 355–367. Web of Science CrossRef CAS IUCr Journals
Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307–326. CrossRef CAS Web of Science
Rawlings, N. D., Morton, F. R., Kok, C. Y., Kong, J. & Barrett, A. J. (2008). Nucleic Acids Res. 36, D320–D325. Web of Science CrossRef PubMed CAS
Sakamoto, W. (2006). Annu. Rev. Plant Biol. 57, 599–621. Web of Science CrossRef PubMed CAS
Schuhmann, H. & Adamska, I. (2012). Physiol. Plant. 145, 224–234. Web of Science CrossRef CAS PubMed
Schuhmann, H., Huesgen, P. F. & Adamska, I. (2012). BMC Plant Biol. 12, 52.
Shan, X., Sun, W., Fan, H., Jia, M., Gao, F. & Gong, W. (2013). Acta Cryst. F69, 69–72. Web of Science CrossRef CAS IUCr Journals
Singh, N., Kuppili, R. R. & Bose, K. (2011). Arch. Biochem. Biophys. 516, 85–96. Web of Science CrossRef CAS PubMed
Sohn, J., Grant, R. A. & Sauer, R. T. (2007). Cell, 131, 572–583. Web of Science CrossRef PubMed CAS
Stael, S., Wurzinger, B., Mair, A., Mehlmer, N., Vothknecht, U. C. & Teige, M. (2012). J. Exp. Bot. 63, 1525–1542. Web of Science CrossRef CAS PubMed
Sun, R., Fan, H., Gao, F., Lin, Y., Zhang, L., Gong, W. & Liu, L. (2012). J. Biol. Chem. 287, 37564–37569. Web of Science CrossRef CAS PubMed
Sun, X., Fu, T., Chen, N., Guo, J., Ma, J., Zou, M., Lu, C. & Zhang, L. (2010). Plant Physiol. 152, 1263–1273. Web of Science CrossRef CAS PubMed
Sun, X., Ouyang, M., Guo, J., Ma, J., Lu, C., Adam, Z. & Zhang, L. (2010). Plant J. 62, 240–249. Web of Science CrossRef CAS PubMed
Sun, X., Peng, L., Guo, J., Chi, W., Ma, J., Lu, C. & Zhang, L. (2007). Plant Cell, 19, 1347–1361. Web of Science CrossRef PubMed CAS
Truebestein, L., Tennstaedt, A., Mönig, T., Krojer, T., Canellas, F., Kaiser, M., Clausen, T. & Ehrmann, M. (2011). Nature Struct. Mol. Biol. 18, 386–388. Web of Science CrossRef CAS
Vagin, A. & Teplyakov, A. (2010). Acta Cryst. D66, 22–25. Web of Science CrossRef CAS IUCr Journals
Voss, N. R. & Gerstein, M. (2010). Nucleic Acids Res. 38, W555–W562. Web of Science CrossRef CAS PubMed
Wilken, C., Kitzing, K., Kurzbauer, R., Ehrmann, M. & Clausen, T. (2004). Cell, 117, 483–494. Web of Science CrossRef PubMed CAS
Winn, M. D. et al. (2011). Acta Cryst. D67, 235–242. Web of Science CrossRef CAS IUCr Journals
Wrase, R., Scott, H., Hilgenfeld, R. & Hansen, G. (2011). Proc. Natl Acad. Sci. USA, 106, 13377–13382.
This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.