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Figure 3
Structure of Deg8 (S292A) in comparison with DegP6 and Deg1 and oligomeric state analysis. (a) Structures of Deg8 (S292A) (green and magenta), DegP6 (PDB entry 1ky9 ; red and orange; the second PDZ domain is not shown for clarity; Krojer et al., 2008BB20) and Deg1 (PDB entry 3qo6 ; cyan and blue). Protease domains and PDZ domains are shown in cartoon and ribbon modes, respectively. (b) The cavity formed in Deg8, DegP6 (PDB entry 1ky9 ; molecule B) and Deg1 (PDB entry 3qo6 ). (c) Structural comparison of protomers of Deg8 (S292A) (green) and Deg1 (PDB entry 3qo6 ; cyan); the difference in PDZ-domain orientations is indicated. (d) Analytical SEC of Deg8 at pH 6.0 (red line) and pH 8.0 (blue line). Different oligomeric states are labelled above the peaks.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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