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Figure 10
2|Fo| − |Fc| nuclear-density map around the carboxyl group of Glu35 of lysozyme at (a) pD = 4.9 and (b) pD = 7.0. Note the pronounced `finger' of nuclear density (see arrow) protruding upwards from atom E35O1 on the left (pD = 4.9) and (b) the lack of such a feature on the carboxylate group on the right (pD = 7.0), that is, the O atom has lost its proton. This suggests that residue E35 of lysozyme is in its protonated (deuterated) form under more active (acidic) conditions. [Note: in both plots, atom E35O1 is circled.]

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