Classifying and assembling two-dimensional X-ray laser diffraction patterns of a single particle to reconstruct the three-dimensional diffraction intensity function: resolution limit due to the quantum noise

Tokuhisa, A.; Taka, J.; Kono, H.; Go, N.

doi:10.1107/S010876731200493X

Acta Crystallographica Section A
Acta Crystallographica
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Figure 1
Examples of two-dimensional diffraction intensity patterns simulated for lysozyme by assuming the wavelength of the incident X-rays $[\lambda]$ = 1 Å and the intensity I_i = 5 × 10²¹ photons pulse⁻¹ mm⁻². In (b), the theoretically expected mean number of diffracted photons arriving at a Shannon pixel is shown. In (a), the number of photons is an integer, chosen according to the Poisson distribution having the theoretically expected mean value as its mean. For this assumed intensity, the mean count of 0.1 photon is observed at $[1/k \cong ]$ 2 Å. In this figure both the abscissa and ordinate show detector coordinates in the sense that they are proportional to coordinates on a flat detector on which the surface of an Ewald sphere is radially projected from its centre (central azimuthal projection).

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ISSN: 2053-2733

Volume 68| Part 3| March 2012| Pages 366-381

doi:10.1107/S010876731200493X

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