Figure 9
Calculated images of immunoglobulin (PDB code 1igt
) on a thin amorphous carbon support (30 Å thick) at 200 kV and CS3 = 0.7 mm. Radiation damage has not been included but in practice would likely prevent this image from being recorded. (a) BF-CTEM, = 520 Å, objective aperture 12 mrad, (b) ABF-STEM, = 520 Å, objective aperture 12 mrad, (c) ABF-STEM, = 365 Å, objective aperture 10.5 mrad, (d) ADF-STEM, = 365 Å, objective aperture 10.5 mrad. Scale bar is 50 Å. |