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Figure 4
Structural analysis of chloroplasts isolated from C. merolae. Light (a) and electron (b) micrographs of a frozen-hydrated chloroplast isolated from C. merolae observed under cryogenic conditions. The sample was frozen on holey carbon film (Quantifoil Micro Tools GmbH, Germany) as in §2[link] and Fig. 2[link](a). Micrographs were taken at 81 K (LM) and 97 K (EM). (c) A diffraction pattern from a frozen-hydrated chloroplast. The pattern is 2 × 2 binned. (d) A projected electron-density map of the chloroplast reconstructed from the diffraction pattern in (c). This was obtained by the HIO–SW PR, but no stable solution was obtained by the OSS PR for this pattern in (c) (see §2[link]). The bars represent 5 µm (a), 1 µm (b) and 500 nm (d). The color scales are shown at the top of the panels in (c) and (d).

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ISSN: 2053-2733
Volume 72| Part 2| March 2016| Pages 179-189
https://doi.org/10.1107/S2053273315023980
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