 | Acta Crystallographica Section A Acta Crystallographica Section A FOUNDATIONS AND ADVANCES |
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![[Figure 5]](mq5039fig5mag.jpg)
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Figure 5
Structural analysis of E. coli minicells. (a) An electron micrograph of a minicell (E. coli strain ME8077) negatively stained with phosphotungstic acid. The arrow indicates a flagellum sticking out from the cell. (b) A diffraction pattern from a frozen-hydrated minicell. The pattern is 2 × 2 binned. (c) A projected electron-density map of the minicell reconstructed from the diffraction pattern shown in (b). Arrows indicate a density similar to the flagellum. (d) An omit difference Fourier map between the observed and calculated structural amplitudes with the calculated phases by masking the flagellum-like density in the reconstructed map. The omit difference Fourier map theoretically gives the density of the omitted structure at half the weight of the original (Drenth, 2007  ). The bars represent 500 nm. The color scales are shown at the top of the panels in (b)–(d). |
![[Figure 5]](mq5039fig5.jpg)
| FOUNDATIONS ADVANCES |
ISSN: 2053-2733
