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Figure 5
Structural analysis of E. coli minicells. (a) An electron micrograph of a minicell (E. coli strain ME8077) negatively stained with phosphotungstic acid. The arrow indicates a flagellum sticking out from the cell. (b) A diffraction pattern from a frozen-hydrated minicell. The pattern is 2 × 2 binned. (c) A projected electron-density map of the minicell reconstructed from the diffraction pattern shown in (b). Arrows indicate a density similar to the flagellum. (d) An omit difference Fourier map between the observed and calculated structural amplitudes with the calculated phases by masking the flagellum-like density in the reconstructed map. The omit difference Fourier map theoretically gives the density of the omitted structure at half the weight of the original (Drenth, 2007 ![]() |