 | Acta Crystallographica Section A Acta Crystallographica Section A FOUNDATIONS AND ADVANCES |
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![[Figure 7]](mq5039fig7mag.jpg)
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Figure 7
Image reconstructions from experimental diffraction patterns of distributed CG particles by the Patterson-search phasing method. (a), (b) Diffraction patterns from CG particles. The patterns are 2 × 2 binned. (c) A sharpened Patterson map calculated from the diffraction pattern shown in (a). The polyhedral nature of CG particles yields `fringes' around the cross-peaks. (d) A superposition minimum function map derived from the sharpened Patterson map in (c) after five iterations (see Methods in Takayama, Maki-Yonekura et al., 2015  ). In the map, correct peaks are found at positions of CG particles, but false peaks that arise from the `fringes' in (c) also appear around the correct peaks. By ignoring the weak peaks which are too close to the strong peaks (closer than 1.5 times the mean diameter of the CG), we assigned circular supports indicated with yellow dotted lines onto the peak positions as an initial support. The diameter of the circle was set to 1.5 times larger than the mean diameter of the CG particles. (e), (f) Projected electron-density maps in (e) and (f) reconstructed from the diffraction patterns in (a) and (b), respectively, by using the Patterson-search phasing method. The whole procedure is semi-automated as described in Takayama, Maki-Yonekura et al. (2015). The bars represent 500 nm. The color scales are identical in the panels in the same row, and shown at the left side of each row. |
![[Figure 7]](mq5039fig7.jpg)
| FOUNDATIONS ADVANCES |
ISSN: 2053-2733
