Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime

Krenkel, M.; Toepperwien, M.; Alves, F.; Salditt, T.

doi:10.1107/S2053273317007902

Figure 1
Improved holo-TIE scheme. (a) Sketch of the algorithmic concept. Multiple images with different Fresnel numbers are recorded, e.g. by changing propagation distances. Distances are chosen such that two images have a small distance $[\Delta z_{1}]$ and two other images have a larger distance $[\Delta z_{2}]$ . (b) The phantom consists of different image components: (A) absorption contrast with 0.76 transmission, (P) phase contrast with 0.55 rad phase shift and (M) mixed contrast of both. (c) Reconstructed phase of the simulated test object (see main text) in the object plane using two images separated by $[\Delta z_{1}]$ . (d) Reconstructed phase in the object plane using two images separated by the larger $[\Delta z_{2}]$ . (e) Phase distribution retrieved using the CTF with homogeneity assumption. Pronounced artifacts near the image components representing pure phase contrast (P) and pure absorption (A), respectively, are observed. (f) Power spectral densities of the original object compared with the multi-distance holo-TIE reconstructions. (g) Multi-distance holo-TIE reconstruction as described in Waller et al. (2010

). (h) Combined multi-distance holo-TIE approach where the detector-plane phase is obtained by a weighted combination of the detector phases from (c) and (d).

FOUNDATIONS
ADVANCES

ISSN: 2053-2733

Volume 73| Part 4| July 2017| Pages 282-292

https://doi.org/10.1107/S2053273317007902

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