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Figure 4
Multi-distance phase retrieval. (a) One representative example out of four processed holograms of a single cell in a defocus series. (b) PSDs of all following reconstructions. (c) CTF reconstruction using all four distances and a homogeneity assumption. (d) Iterative HIO reconstruction with support and pure-phase constraints. (e) Phase distribution in the object plane retrieved with the combined holo-TIE scheme using two intensity images, where the low-frequency image was created using the CTF guess. (f) IRGN reconstruction using all four measurement planes with a pure-phase assumption, but without any support constraint. From Krenkel (2015BB21). All scale bars denote 5 µm.

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