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Figure 5
Tomography result of an isolated macrophage which was labelled with barium sulfate and osmium tetroxide. (a) Orthoslice through the plane perpendicular to the tomographic axis. Besides the cell with its internal contrast particles, the Petri dish and the resin used for embedding can be resolved too. (b) Orthoslice coplanar to the projection direction. (c) Three-dimensional rendering of the data set, showing barium particles in green and the homogeneous osmium inside the cell in half-transparent blue. Phase retrieval for all projections was carried out using the CTF approach with four distances, followed by a tomographic alignment procedure. Scale bars denote 5 µm.

ISSN: 2053-2733
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