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Figure 6
Phase distributions of macrophages in PBS, labelled with gold-enhanced antibodies (anti-CD68). The holograms were recorded under non-optimal illumination conditions, compromising the quality of the phase retrieval. (a) Phase distribution reconstructed using the CTF-based multi-distance approach, which amplifies the artifacts present in the hologram. (b) Iterative multi-distance HIO reconstruction, where a manually determined support area is enforced, sufficient to suppress the artifacts. From Krenkel (2015BB21). Scale bars denote 5 µm.

ISSN: 2053-2733
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